Quercetin and alendronate multi-functionalized materials as tools to hinder oxidative stress damage.

Lucia Forte, Paola Torricelli, Elisa Boanini, Rubini Katia, Milena Fini, Adriana Bigi

Research output: Contribution to journalArticle

Abstract

In spite of its remarkable anti-oxidant, anti-inflammatory, anti-cancer properties and its possible inhibition activity towards bone resorption, quercetin therapeutic use is limited by its poor bioavailability. Herein we developed a new multifunctionalized system for the local administration of quercetin and alendronate, one of the most potent anti-osteoporotic drugs, with the aim to get a material with enhanced properties. To this purpose we loaded quercetin on hydroxyapatite functionalized with alendronate (HAAL), as well as on hydroxyapatite (HA). Characterization was performed by means of X-ray diffraction, FT-IR and Raman spectroscopies, thermogravimetric and spectrophotometric analyses. Loading of quercetin from hydro-alcoholic solution increased with time and reached a constant value of about 5 weight% on both substrates, without causing significant structural and morphological modifications. Quercetin functionalized materials exhibit relevant anti-oxidant properties, in agreement with their high radical scavenging activity, and a quercetin sustained release in phosphate buffer. In vitro osteoblast and osteoclast co-culture in a microenvironment altered by oxidative stress shows that both alendronate and quercetin significantly reduce osteoclast viability, whereas they are able to counteract the negative effect of oxidative stress on osteoblast viability and differentiation, suggesting that their relative amount in the functionalized materials can be utilized to tailor bone cells response. This article is protected by copyright. All rights reserved.
Original languageEnglish
Pages (from-to)3293-3303
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume105
Issue number12
DOIs
Publication statusPublished - 2018

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Alendronate
Oxidative stress
Quercetin
Osteoblasts
Oxidants
Hydroxyapatite
Bone
Scavenging
Cell culture
Durapatite
Raman spectroscopy
Infrared spectroscopy
Phosphates
X ray diffraction
Substrates
Buffers
Anti-Inflammatory Agents
Osteoclasts
Pharmaceutical Preparations

Keywords

  • alendronate
  • hydroxyapatite
  • osteoblast
  • osteoclast
  • oxidative stress
  • quercetin

Cite this

Quercetin and alendronate multi-functionalized materials as tools to hinder oxidative stress damage. / Forte, Lucia; Torricelli, Paola; Boanini, Elisa; Katia, Rubini; Fini, Milena; Bigi, Adriana.

In: Journal of Biomedical Materials Research - Part A, Vol. 105, No. 12, 2018, p. 3293-3303.

Research output: Contribution to journalArticle

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AB - In spite of its remarkable anti-oxidant, anti-inflammatory, anti-cancer properties and its possible inhibition activity towards bone resorption, quercetin therapeutic use is limited by its poor bioavailability. Herein we developed a new multifunctionalized system for the local administration of quercetin and alendronate, one of the most potent anti-osteoporotic drugs, with the aim to get a material with enhanced properties. To this purpose we loaded quercetin on hydroxyapatite functionalized with alendronate (HAAL), as well as on hydroxyapatite (HA). Characterization was performed by means of X-ray diffraction, FT-IR and Raman spectroscopies, thermogravimetric and spectrophotometric analyses. Loading of quercetin from hydro-alcoholic solution increased with time and reached a constant value of about 5 weight% on both substrates, without causing significant structural and morphological modifications. Quercetin functionalized materials exhibit relevant anti-oxidant properties, in agreement with their high radical scavenging activity, and a quercetin sustained release in phosphate buffer. In vitro osteoblast and osteoclast co-culture in a microenvironment altered by oxidative stress shows that both alendronate and quercetin significantly reduce osteoclast viability, whereas they are able to counteract the negative effect of oxidative stress on osteoblast viability and differentiation, suggesting that their relative amount in the functionalized materials can be utilized to tailor bone cells response. This article is protected by copyright. All rights reserved.

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