Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells

Enrico Lugli, Roberta Ferraresi, Erika Roat, Leonarda Troiano, Marcello Pinti, Milena Nasi, Elisa Nemes, Linda Bertoncelli, Lara Gibellini, Paolo Salomoni, Edwin L. Cooper, Andrea Cossarizza

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Toxicity of chemotherapeutic drugs towards normal cells is a serious side effect of cancer treatment. Thus, finding of molecules with low toxicity for normal cells is crucial. Several natural compounds, such as flavonoid quercertin, are receiving a growing attention as "chemopreventers". Quercetin kills tumour-derived cell lines, but little is known about its effects on normal cells. Here we show that although quercetin exerts a higher apoptotic potential on leukemic cell lines than on peripheral blood mononuclear cells (PBMCs) and does not sensitize PBMCs to CD95-induced apoptosis, it is able to inhibit normal immune functions such as T cell proliferation and activation. Quercetin sensitivity is independent on cell cycle progression since it was not abrogated in serum-starved U937 cells, nor proliferating PBMCs underwent apoptosis after quercetin treatment. However, quercetin prevented PHA-induced PBMC proliferation and SEB-induced upregulation of activation markers. Our data suggest that quercetin, while incapable of inducing apoptosis in normal cells under several conditions, could interfere with effector T cell function.

Original languageEnglish
Pages (from-to)140-150
Number of pages11
JournalLeukemia Research
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Quercetin
Lymphocyte Activation
Apoptosis
Blood Cells
Cell Proliferation
T-Lymphocytes
U937 Cells
Tumor Cell Line
Drug-Related Side Effects and Adverse Reactions
Flavonoids
Cell Cycle
Up-Regulation
Cell Line
Serum
Neoplasms

Keywords

  • Apoptosis
  • Leukemic cell lines
  • Lymphocyte function
  • PBMCs
  • Quercetin

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells. / Lugli, Enrico; Ferraresi, Roberta; Roat, Erika; Troiano, Leonarda; Pinti, Marcello; Nasi, Milena; Nemes, Elisa; Bertoncelli, Linda; Gibellini, Lara; Salomoni, Paolo; Cooper, Edwin L.; Cossarizza, Andrea.

In: Leukemia Research, Vol. 33, No. 1, 01.2009, p. 140-150.

Research output: Contribution to journalArticle

Lugli, E, Ferraresi, R, Roat, E, Troiano, L, Pinti, M, Nasi, M, Nemes, E, Bertoncelli, L, Gibellini, L, Salomoni, P, Cooper, EL & Cossarizza, A 2009, 'Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells', Leukemia Research, vol. 33, no. 1, pp. 140-150. https://doi.org/10.1016/j.leukres.2008.07.025
Lugli, Enrico ; Ferraresi, Roberta ; Roat, Erika ; Troiano, Leonarda ; Pinti, Marcello ; Nasi, Milena ; Nemes, Elisa ; Bertoncelli, Linda ; Gibellini, Lara ; Salomoni, Paolo ; Cooper, Edwin L. ; Cossarizza, Andrea. / Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells. In: Leukemia Research. 2009 ; Vol. 33, No. 1. pp. 140-150.
@article{ca18c0196435461a9b050ff4e406ac4d,
title = "Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells",
abstract = "Toxicity of chemotherapeutic drugs towards normal cells is a serious side effect of cancer treatment. Thus, finding of molecules with low toxicity for normal cells is crucial. Several natural compounds, such as flavonoid quercertin, are receiving a growing attention as {"}chemopreventers{"}. Quercetin kills tumour-derived cell lines, but little is known about its effects on normal cells. Here we show that although quercetin exerts a higher apoptotic potential on leukemic cell lines than on peripheral blood mononuclear cells (PBMCs) and does not sensitize PBMCs to CD95-induced apoptosis, it is able to inhibit normal immune functions such as T cell proliferation and activation. Quercetin sensitivity is independent on cell cycle progression since it was not abrogated in serum-starved U937 cells, nor proliferating PBMCs underwent apoptosis after quercetin treatment. However, quercetin prevented PHA-induced PBMC proliferation and SEB-induced upregulation of activation markers. Our data suggest that quercetin, while incapable of inducing apoptosis in normal cells under several conditions, could interfere with effector T cell function.",
keywords = "Apoptosis, Leukemic cell lines, Lymphocyte function, PBMCs, Quercetin",
author = "Enrico Lugli and Roberta Ferraresi and Erika Roat and Leonarda Troiano and Marcello Pinti and Milena Nasi and Elisa Nemes and Linda Bertoncelli and Lara Gibellini and Paolo Salomoni and Cooper, {Edwin L.} and Andrea Cossarizza",
year = "2009",
month = "1",
doi = "10.1016/j.leukres.2008.07.025",
language = "English",
volume = "33",
pages = "140--150",
journal = "Leukemia Research",
issn = "0145-2126",
publisher = "Elsevier Ltd",
number = "1",

}

TY - JOUR

T1 - Quercetin inhibits lymphocyte activation and proliferation without inducing apoptosis in peripheral mononuclear cells

AU - Lugli, Enrico

AU - Ferraresi, Roberta

AU - Roat, Erika

AU - Troiano, Leonarda

AU - Pinti, Marcello

AU - Nasi, Milena

AU - Nemes, Elisa

AU - Bertoncelli, Linda

AU - Gibellini, Lara

AU - Salomoni, Paolo

AU - Cooper, Edwin L.

AU - Cossarizza, Andrea

PY - 2009/1

Y1 - 2009/1

N2 - Toxicity of chemotherapeutic drugs towards normal cells is a serious side effect of cancer treatment. Thus, finding of molecules with low toxicity for normal cells is crucial. Several natural compounds, such as flavonoid quercertin, are receiving a growing attention as "chemopreventers". Quercetin kills tumour-derived cell lines, but little is known about its effects on normal cells. Here we show that although quercetin exerts a higher apoptotic potential on leukemic cell lines than on peripheral blood mononuclear cells (PBMCs) and does not sensitize PBMCs to CD95-induced apoptosis, it is able to inhibit normal immune functions such as T cell proliferation and activation. Quercetin sensitivity is independent on cell cycle progression since it was not abrogated in serum-starved U937 cells, nor proliferating PBMCs underwent apoptosis after quercetin treatment. However, quercetin prevented PHA-induced PBMC proliferation and SEB-induced upregulation of activation markers. Our data suggest that quercetin, while incapable of inducing apoptosis in normal cells under several conditions, could interfere with effector T cell function.

AB - Toxicity of chemotherapeutic drugs towards normal cells is a serious side effect of cancer treatment. Thus, finding of molecules with low toxicity for normal cells is crucial. Several natural compounds, such as flavonoid quercertin, are receiving a growing attention as "chemopreventers". Quercetin kills tumour-derived cell lines, but little is known about its effects on normal cells. Here we show that although quercetin exerts a higher apoptotic potential on leukemic cell lines than on peripheral blood mononuclear cells (PBMCs) and does not sensitize PBMCs to CD95-induced apoptosis, it is able to inhibit normal immune functions such as T cell proliferation and activation. Quercetin sensitivity is independent on cell cycle progression since it was not abrogated in serum-starved U937 cells, nor proliferating PBMCs underwent apoptosis after quercetin treatment. However, quercetin prevented PHA-induced PBMC proliferation and SEB-induced upregulation of activation markers. Our data suggest that quercetin, while incapable of inducing apoptosis in normal cells under several conditions, could interfere with effector T cell function.

KW - Apoptosis

KW - Leukemic cell lines

KW - Lymphocyte function

KW - PBMCs

KW - Quercetin

UR - http://www.scopus.com/inward/record.url?scp=55049133406&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55049133406&partnerID=8YFLogxK

U2 - 10.1016/j.leukres.2008.07.025

DO - 10.1016/j.leukres.2008.07.025

M3 - Article

C2 - 18774171

AN - SCOPUS:55049133406

VL - 33

SP - 140

EP - 150

JO - Leukemia Research

JF - Leukemia Research

SN - 0145-2126

IS - 1

ER -