Quinolone Resistance in Absence of Selective Pressure: The Experience of a Very Remote Community in the Amazon Forest

Lucia Pallecchi, Alessandro Bartoloni, Eleonora Riccobono, Connie Fernandez, Antonia Mantella, Donata Magnelli, Dario Mannini, Marianne Strohmeyer, Filippo Bartalesi, Hugo Rodriguez, Eduardo Gotuzzo, Gian Maria Rossolini

Research output: Contribution to journalArticle

Abstract

Background: Quinolones are potent broad-spectrum bactericidal agents increasingly employed also in resource-limited countries. Resistance to quinolones is an increasing problem, known to be strongly associated with quinolone exposure. We report on the emergence of quinolone resistance in a very remote community in the Amazon forest, where quinolones have never been used and quinolone resistance was absent in 2002. Methods: The community exhibited a considerable level of geographical isolation, limited contact with the exterior and minimal antibiotic use (not including quinolones). In December 2009, fecal carriage of antibiotic resistant Escherichia coli was investigated in 120 of the 140 inhabitants, and in 48 animals reared in the community. All fluoroquinolone-resistant isolates were genotyped and characterized for the mechanisms of plasmid- and chromosomal-mediated quinolone resistance. Principal Findings: Despite the characteristics of the community remained substantially unchanged during the period 2002-2009, carriage of quinolone-resistant E. coli was found to be common in 2009 both in humans (45% nalidixic acid, 14% ciprofloxacin) and animals (54% nalidixic acid, 23% ciprofloxacin). Ciprofloxacin-resistant isolates of human and animal origin showed multidrug resistance phenotypes, a high level of genetic heterogeneity, and a combination of GyrA (Ser83Leu and Asp87Asn) and ParC (Ser80Ile) substitutions commonly observed in fluoroquinolone-resistant clinical isolates of E. coli. Conclusions: Remoteness and absence of antibiotic selective pressure did not protect the community from the remarkable emergence of quinolone resistance in E. coli. Introduction of the resistant strains from antibiotic-exposed settings is the most likely source, while persistence and dissemination in the absence of quinolone exposure is likely mostly related with poor sanitation. Interventions aimed at reducing the spreading of resistant isolates (by improving sanitation and water/food safety) are urgently needed to preserve the efficacy of quinolones in resource-limited countries, as control strategies based only on antibiotic restriction policies are unlikely to succeed in those settings.

Original languageEnglish
Article numbere1790
JournalPLoS Neglected Tropical Diseases
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 2012

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Quinolones
Anti-Bacterial Agents
Ciprofloxacin
Escherichia coli
Nalidixic Acid
Sanitation
Fluoroquinolones
Forests
Genetic Heterogeneity
Food Safety
Multiple Drug Resistance
Plasmids

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Pallecchi, L., Bartoloni, A., Riccobono, E., Fernandez, C., Mantella, A., Magnelli, D., ... Rossolini, G. M. (2012). Quinolone Resistance in Absence of Selective Pressure: The Experience of a Very Remote Community in the Amazon Forest. PLoS Neglected Tropical Diseases, 6(8), [e1790]. https://doi.org/10.1371/journal.pntd.0001790

Quinolone Resistance in Absence of Selective Pressure : The Experience of a Very Remote Community in the Amazon Forest. / Pallecchi, Lucia; Bartoloni, Alessandro; Riccobono, Eleonora; Fernandez, Connie; Mantella, Antonia; Magnelli, Donata; Mannini, Dario; Strohmeyer, Marianne; Bartalesi, Filippo; Rodriguez, Hugo; Gotuzzo, Eduardo; Rossolini, Gian Maria.

In: PLoS Neglected Tropical Diseases, Vol. 6, No. 8, e1790, 08.2012.

Research output: Contribution to journalArticle

Pallecchi, L, Bartoloni, A, Riccobono, E, Fernandez, C, Mantella, A, Magnelli, D, Mannini, D, Strohmeyer, M, Bartalesi, F, Rodriguez, H, Gotuzzo, E & Rossolini, GM 2012, 'Quinolone Resistance in Absence of Selective Pressure: The Experience of a Very Remote Community in the Amazon Forest', PLoS Neglected Tropical Diseases, vol. 6, no. 8, e1790. https://doi.org/10.1371/journal.pntd.0001790
Pallecchi, Lucia ; Bartoloni, Alessandro ; Riccobono, Eleonora ; Fernandez, Connie ; Mantella, Antonia ; Magnelli, Donata ; Mannini, Dario ; Strohmeyer, Marianne ; Bartalesi, Filippo ; Rodriguez, Hugo ; Gotuzzo, Eduardo ; Rossolini, Gian Maria. / Quinolone Resistance in Absence of Selective Pressure : The Experience of a Very Remote Community in the Amazon Forest. In: PLoS Neglected Tropical Diseases. 2012 ; Vol. 6, No. 8.
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