Quorum sensing inhibitor FS3-coated vascular graft enhances daptomycin efficacy in a rat model of staphylococcal infection

Oscar Cirioni, Federico Mocchegiani, Ivana Cacciatore, Jacopo Vecchiet, Carmela Silvestri, Leonardo Baldassarre, Claudio Ucciferri, Elena Orsetti, Pamela Castelli, Mauro Provinciali, Marco Vivarelli, Erika Fornasari, Andrea Giacometti

Research output: Contribution to journalArticle

Abstract

The aim of the study was to investigate the efficacy of the quorum sensing inhibitor FS3 and daptomycin in preventing prosthesis biofilm in a rat model of staphylococcal vascular graft infection. Graft infections were established in the back subcutaneous tissue of adult male Wistar rats by implantation of Dacron prostheses followed by topical inoculation with 2 × 107 colony-forming units of Staphylococcus aureus, strain Smith diffuse. The study included a control group, a contaminated group that did not receive any antibiotic prophylaxis and three contaminated groups that received: (i) intraperitoneal daptomycin, (ii) FS3-soacked graft, and (iii) daptomycin plus FS3-soaked graft, respectively. Each group included 15 animals. The infection burden was evaluated by using sonication and quantitative agar culture. Moreover, an in vitro binding-study was performed to quantify the how much FS3 was coated to the surface of the prosthesis. The in vitro studies showed, that minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values for daptomycin were lower in presence of FS3. In in vivo studies, when tested alone, daptomycin and FS3 showed good efficacies. Their combination showed efficacies significantly higher than that of each single compound. Daptomycin is an important candidate for prevention of staphylococcal biofilm related infection and FS3 could serve as an interesting anti-staphylococcal antibiotic enhancer.

Original languageEnglish
Pages (from-to)77-81
Number of pages5
JournalPeptides
Volume40
DOIs
Publication statusPublished - Feb 2013

Keywords

  • Bacterial biofilm
  • Daptomycin
  • FS3
  • Lipopeptides
  • Vascular graft infection

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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    Cirioni, O., Mocchegiani, F., Cacciatore, I., Vecchiet, J., Silvestri, C., Baldassarre, L., Ucciferri, C., Orsetti, E., Castelli, P., Provinciali, M., Vivarelli, M., Fornasari, E., & Giacometti, A. (2013). Quorum sensing inhibitor FS3-coated vascular graft enhances daptomycin efficacy in a rat model of staphylococcal infection. Peptides, 40, 77-81. https://doi.org/10.1016/j.peptides.2012.12.002