Rabbit extracellular superoxide dismutase: Expression and effect on LDL oxidation

Mikko O. Laukkanen, Pauliina Lehtolainen, Päivi Turunen, Saara Aittomäki, Pekka Oikari, Stefan L. Marklund, Seppo Ylä-Herttuala

Research output: Contribution to journalArticle

Abstract

Extracellular superoxide dismutase (EC-SOD) is a secreted antioxidative enzyme with an abundant mRNA expression in kidney and arterial wall. In order to study expression and antioxidative function of EC-SOD, we cloned the rabbit ec-sod cDNA and produced the recombinant protein in cell culture. In vitro studies did not show a direct relationship between the amounts of synthesized mRNA and secreted protein activity, suggesting post- transcriptional regulation. The antiatherogenic role of EC-SOD was studied by determining the effect of EC-SOD on the oxidation (ox) of low density lipoprotein (LDL), and subsequent degradation of oxLDL in RAW 264 macrophages in vitro. It was found that recombinant EC-SOD reduced both the degradation of LDL in RAW 264 macrophages by 28-36% and its electrophoretic mobility caused by endothelial cell-mediated oxidation. It is therefore suggested that EC-SOD can act as a protective enzyme against the development of atherosclerosis. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)173-179
Number of pages7
JournalGene
Volume254
Issue number1-2
DOIs
Publication statusPublished - Aug 22 2000

Keywords

  • Adenovirus
  • Antioxidant
  • EC-SOD
  • Post-transcriptional regulation

ASJC Scopus subject areas

  • Genetics

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    Laukkanen, M. O., Lehtolainen, P., Turunen, P., Aittomäki, S., Oikari, P., Marklund, S. L., & Ylä-Herttuala, S. (2000). Rabbit extracellular superoxide dismutase: Expression and effect on LDL oxidation. Gene, 254(1-2), 173-179. https://doi.org/10.1016/S0378-1119(00)00272-9