TY - JOUR
T1 - Rabbit extracellular superoxide dismutase
T2 - Expression and effect on LDL oxidation
AU - Laukkanen, Mikko O.
AU - Lehtolainen, Pauliina
AU - Turunen, Päivi
AU - Aittomäki, Saara
AU - Oikari, Pekka
AU - Marklund, Stefan L.
AU - Ylä-Herttuala, Seppo
PY - 2000/8/22
Y1 - 2000/8/22
N2 - Extracellular superoxide dismutase (EC-SOD) is a secreted antioxidative enzyme with an abundant mRNA expression in kidney and arterial wall. In order to study expression and antioxidative function of EC-SOD, we cloned the rabbit ec-sod cDNA and produced the recombinant protein in cell culture. In vitro studies did not show a direct relationship between the amounts of synthesized mRNA and secreted protein activity, suggesting post- transcriptional regulation. The antiatherogenic role of EC-SOD was studied by determining the effect of EC-SOD on the oxidation (ox) of low density lipoprotein (LDL), and subsequent degradation of oxLDL in RAW 264 macrophages in vitro. It was found that recombinant EC-SOD reduced both the degradation of LDL in RAW 264 macrophages by 28-36% and its electrophoretic mobility caused by endothelial cell-mediated oxidation. It is therefore suggested that EC-SOD can act as a protective enzyme against the development of atherosclerosis. (C) 2000 Elsevier Science B.V.
AB - Extracellular superoxide dismutase (EC-SOD) is a secreted antioxidative enzyme with an abundant mRNA expression in kidney and arterial wall. In order to study expression and antioxidative function of EC-SOD, we cloned the rabbit ec-sod cDNA and produced the recombinant protein in cell culture. In vitro studies did not show a direct relationship between the amounts of synthesized mRNA and secreted protein activity, suggesting post- transcriptional regulation. The antiatherogenic role of EC-SOD was studied by determining the effect of EC-SOD on the oxidation (ox) of low density lipoprotein (LDL), and subsequent degradation of oxLDL in RAW 264 macrophages in vitro. It was found that recombinant EC-SOD reduced both the degradation of LDL in RAW 264 macrophages by 28-36% and its electrophoretic mobility caused by endothelial cell-mediated oxidation. It is therefore suggested that EC-SOD can act as a protective enzyme against the development of atherosclerosis. (C) 2000 Elsevier Science B.V.
KW - Adenovirus
KW - Antioxidant
KW - EC-SOD
KW - Post-transcriptional regulation
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U2 - 10.1016/S0378-1119(00)00272-9
DO - 10.1016/S0378-1119(00)00272-9
M3 - Article
C2 - 10974548
AN - SCOPUS:0034702876
VL - 254
SP - 173
EP - 179
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -