Rabbit monoclonal Fab derived from a phage display library

Maria Foti, Francesca Granucci, Paola Ricciardi-Castagnoli, Adriano Spreafico, Mathias Ackermann, Mark Suter

Research output: Contribution to journalArticle

Abstract

Rabbit monoclonal antibodies (RmAb) are not routinely obtained by eukaryotic cell fusion techniques. Therefore, we have applied phage display technology to produce a recombinant rabbit Fab molecule directed against the KLH model antigen. The Fab fragments selected from the rabbit phage display library were subcloned in an expression vector to permit the production of a fusion protein comprising a dimer of bacterial alkaline phosphatase (phoA). This fusion protein was directly produced into the periplasmic space of Escherichia coli. We show that a crude extract containing these conjugates can be used in a direct enzyme immunoassay, as exemplified in the case of the KLH antigen.

Original languageEnglish
Pages (from-to)201-212
Number of pages12
JournalJournal of Immunological Methods
Volume213
Issue number2
DOIs
Publication statusPublished - Jun 1998

Fingerprint

Bacteriophages
Libraries
Rabbits
Periplasm
Immunoglobulin Fab Fragments
Cell Fusion
Eukaryotic Cells
Complex Mixtures
Immunoenzyme Techniques
Alkaline Phosphatase
Proteins
Monoclonal Antibodies
Escherichia coli
Technology
keyhole-limpet hemocyanin

Keywords

  • Alkaline phosphatase
  • Fab fragments
  • Phage display
  • Rabbit immunoglobulins
  • Recombinant antibody

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Foti, M., Granucci, F., Ricciardi-Castagnoli, P., Spreafico, A., Ackermann, M., & Suter, M. (1998). Rabbit monoclonal Fab derived from a phage display library. Journal of Immunological Methods, 213(2), 201-212. https://doi.org/10.1016/S0022-1759(98)00029-5

Rabbit monoclonal Fab derived from a phage display library. / Foti, Maria; Granucci, Francesca; Ricciardi-Castagnoli, Paola; Spreafico, Adriano; Ackermann, Mathias; Suter, Mark.

In: Journal of Immunological Methods, Vol. 213, No. 2, 06.1998, p. 201-212.

Research output: Contribution to journalArticle

Foti, M, Granucci, F, Ricciardi-Castagnoli, P, Spreafico, A, Ackermann, M & Suter, M 1998, 'Rabbit monoclonal Fab derived from a phage display library', Journal of Immunological Methods, vol. 213, no. 2, pp. 201-212. https://doi.org/10.1016/S0022-1759(98)00029-5
Foti M, Granucci F, Ricciardi-Castagnoli P, Spreafico A, Ackermann M, Suter M. Rabbit monoclonal Fab derived from a phage display library. Journal of Immunological Methods. 1998 Jun;213(2):201-212. https://doi.org/10.1016/S0022-1759(98)00029-5
Foti, Maria ; Granucci, Francesca ; Ricciardi-Castagnoli, Paola ; Spreafico, Adriano ; Ackermann, Mathias ; Suter, Mark. / Rabbit monoclonal Fab derived from a phage display library. In: Journal of Immunological Methods. 1998 ; Vol. 213, No. 2. pp. 201-212.
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