Rabbit monoclonal Fab derived from a phage display library

Maria Foti; Francesca Granucci; Paola Ricciardi-Castagnoli; Adriano Spreafico; Mathias Ackermann; Mark Suter

doi:10.1016/S0022-1759(98)00029-5

Rabbit monoclonal Fab derived from a phage display library

Maria Foti, Francesca Granucci, Paola Ricciardi-Castagnoli, Adriano Spreafico, Mathias Ackermann, Mark Suter

Istituto Clinico Humanitas

Research output: Contribution to journal › Article › peer-review

Abstract

Rabbit monoclonal antibodies (RmAb) are not routinely obtained by eukaryotic cell fusion techniques. Therefore, we have applied phage display technology to produce a recombinant rabbit Fab molecule directed against the KLH model antigen. The Fab fragments selected from the rabbit phage display library were subcloned in an expression vector to permit the production of a fusion protein comprising a dimer of bacterial alkaline phosphatase (phoA). This fusion protein was directly produced into the periplasmic space of Escherichia coli. We show that a crude extract containing these conjugates can be used in a direct enzyme immunoassay, as exemplified in the case of the KLH antigen.

Original language	English
Pages (from-to)	201-212
Number of pages	12
Journal	Journal of Immunological Methods
Volume	213
Issue number	2
DOIs	https://doi.org/10.1016/S0022-1759(98)00029-5
Publication status	Published - Jun 1998

Keywords

Alkaline phosphatase
Fab fragments
Phage display
Rabbit immunoglobulins
Recombinant antibody

ASJC Scopus subject areas

Biotechnology
Immunology

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10.1016/S0022-1759(98)00029-5

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title = "Rabbit monoclonal Fab derived from a phage display library",

abstract = "Rabbit monoclonal antibodies (RmAb) are not routinely obtained by eukaryotic cell fusion techniques. Therefore, we have applied phage display technology to produce a recombinant rabbit Fab molecule directed against the KLH model antigen. The Fab fragments selected from the rabbit phage display library were subcloned in an expression vector to permit the production of a fusion protein comprising a dimer of bacterial alkaline phosphatase (phoA). This fusion protein was directly produced into the periplasmic space of Escherichia coli. We show that a crude extract containing these conjugates can be used in a direct enzyme immunoassay, as exemplified in the case of the KLH antigen.",

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AU - Foti, Maria

AU - Granucci, Francesca

AU - Ricciardi-Castagnoli, Paola

AU - Spreafico, Adriano

AU - Ackermann, Mathias

AU - Suter, Mark

PY - 1998/6

Y1 - 1998/6

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AB - Rabbit monoclonal antibodies (RmAb) are not routinely obtained by eukaryotic cell fusion techniques. Therefore, we have applied phage display technology to produce a recombinant rabbit Fab molecule directed against the KLH model antigen. The Fab fragments selected from the rabbit phage display library were subcloned in an expression vector to permit the production of a fusion protein comprising a dimer of bacterial alkaline phosphatase (phoA). This fusion protein was directly produced into the periplasmic space of Escherichia coli. We show that a crude extract containing these conjugates can be used in a direct enzyme immunoassay, as exemplified in the case of the KLH antigen.

KW - Alkaline phosphatase

KW - Fab fragments

KW - Phage display

KW - Rabbit immunoglobulins

KW - Recombinant antibody

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