Rac3-induced neuritogenesis requires binding to neurabin I

Donata Orioli, Ivan N. Colaluca, Miria Stefanini, Silvano Riva, Carlos G. Dotti, Fiorenzo A. Peverali

Research output: Contribution to journalArticlepeer-review

Abstract

Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.

Original languageEnglish
Pages (from-to)2391-2400
Number of pages10
JournalMolecular Biology of the Cell
Volume17
Issue number5
DOIs
Publication statusPublished - May 2006

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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