RACK-1 expression and cytokine production in leukocytes obtained from AD patients

Marco Racchi, Elena Sinforiani, Stefano Govoni, Marina Marinovich, Corrado L. Galli, Emanuela Corsini

Research output: Contribution to journalArticle

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Abstract

Background and aims: The purpose of this study was to evaluate in vitro cytokine production in blood leukocytes obtained from sporadic AD patients, aged controls and young individuals. Methods: Diluted whole blood was treated in the presence or absence of LPS (1 μg/mL) for varying times (3-48 h). The release of IL-8, IL-10 and TNF-α in conditioned media was evaluated by commercially available sandwich ELISA. Results: Data obtained are indicative of the presence of an unregulated systemic inflammation in AD patients. Leukocytes obtained from AD patients had increased spontaneous TNF-α release and decreased LPS-induced IL-10 production, in comparison with both old controls and young subjects, while identical IL-8 production was observed in all groups. The last finding indicates that there was no shift in the potency or efficacy of the response towards LPS with aging, but alterations in downstream signal transduction pathways are probably altered with aging and pathological conditions. Conclusions: The dysreg ulation of cytokine production observed in AD patients may partially be explained by a significant reduction in the expression of RACK-1, a protein crucial for integration of signaling pathways with different physiological functions, such as cytokine production.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalAging clinical and experimental research
Volume18
Issue number2
Publication statusPublished - Apr 2006

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Leukocytes
Cytokines
Interleukin-8
Interleukin-10
Conditioned Culture Medium
Signal Transduction
Enzyme-Linked Immunosorbent Assay
Inflammation
Proteins

Keywords

  • Aging
  • Cytokine
  • DHEA
  • Immunosenescence
  • Protein kinase C

ASJC Scopus subject areas

  • Ageing

Cite this

Racchi, M., Sinforiani, E., Govoni, S., Marinovich, M., Galli, C. L., & Corsini, E. (2006). RACK-1 expression and cytokine production in leukocytes obtained from AD patients. Aging clinical and experimental research, 18(2), 153-157.

RACK-1 expression and cytokine production in leukocytes obtained from AD patients. / Racchi, Marco; Sinforiani, Elena; Govoni, Stefano; Marinovich, Marina; Galli, Corrado L.; Corsini, Emanuela.

In: Aging clinical and experimental research, Vol. 18, No. 2, 04.2006, p. 153-157.

Research output: Contribution to journalArticle

Racchi, M, Sinforiani, E, Govoni, S, Marinovich, M, Galli, CL & Corsini, E 2006, 'RACK-1 expression and cytokine production in leukocytes obtained from AD patients', Aging clinical and experimental research, vol. 18, no. 2, pp. 153-157.
Racchi, Marco ; Sinforiani, Elena ; Govoni, Stefano ; Marinovich, Marina ; Galli, Corrado L. ; Corsini, Emanuela. / RACK-1 expression and cytokine production in leukocytes obtained from AD patients. In: Aging clinical and experimental research. 2006 ; Vol. 18, No. 2. pp. 153-157.
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