Radiation effects on development of HER2-positive breast carcinomas

Fabio Castiglioni, Monica Terenziani, Maria Luisa Carcangiu, Raffaella Miliano, Piera Aiello, Lorenzo Bertola, Tiziana Triulzi, Patrizia Gasparini, Tiziana Camerini, Gabriella Sozzi, Franca Fossati-Bellani, Sylvie Ménard, Elda Tagliabue

Research output: Contribution to journalArticle

Abstract

Purpose: Neither hormone-related nor genetics risk factors have been associated with the development of highly proliferative HER2-positive breast carcinomas. Because the majority of HER2-positive tumors present the amplification of the oncogene, we asked whether genomic instability triggered by irradiation might be involved in the induction of HER2-overexpressing breast carcinomas. Experimental Design: Sixty-six infiltrating breast carcinomas from patients treated with radiation therapy for Hodgkin's lymphoma or other pediatric solid tumors and a control series of 61 consecutive sporadic breast tumors were analyzed by immunohistochemistry for HER2 expression with HercepTest. A panel of antibodies against estrogen receptor, progesterone receptor, c-kit, cytokeratin 5/6, p53, and ki67 antigen was also used to identify differentiation subsets and molecular characteristics of the analyzed breast carcinomas. Results: Although no differences between the two tumor series were found with respect to HER2 expression scored 2+ and 3+, the percentage of 3+ HER2-positive tumors was significantly higher in patients irradiated during breast maturation compared with patients irradiated after breast maturation (35.3% versus 12.5%, P = 0.046). In the latter group, 52.5% of the breast carcinomas showed basal-like differentiation (estrogen receptor, progesterone receptor, and HER2 negative) versus only 5.9% in the group irradiated during breast development (P <0.0001). Analysis adjusted for age confirmed the significant increase in basal-like tumor development in patients irradiated within 4 years of menarche, but also showed that the differences between patients irradiated before and after puberty in HER2 3+ tumor frequencies are due to age-related differences in HER2 3+ tumor onset. Conclusion: Together, our data indicate that the development of HER2-positive tumors correlates with timing rather than type of carcinogenic hits and provide clear evidence that radiation is a risk factor for breast carcinomas showing basal-like differentiation.

Original languageEnglish
Pages (from-to)46-51
Number of pages6
JournalClinical Cancer Research
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Castiglioni, F., Terenziani, M., Carcangiu, M. L., Miliano, R., Aiello, P., Bertola, L., Triulzi, T., Gasparini, P., Camerini, T., Sozzi, G., Fossati-Bellani, F., Ménard, S., & Tagliabue, E. (2007). Radiation effects on development of HER2-positive breast carcinomas. Clinical Cancer Research, 13(1), 46-51. https://doi.org/10.1158/1078-0432.CCR-06-1490