Abstract
Background: Recent work has identified a category of genes devoted to the control of genomic stability and prevention of cellular evolution. They encode components of cell cycle checkpoint, i.e., regulatory pathways committed to ordered cell cycle transition and fidelity of replicated DNA under adverse environmental conditions, such as those following exposure to genotoxic agents. Gadd45 belongs to the class II family of DNA damage- inducible (DDI) gene, and its role in DNA repair has been proved in many experimental models. The aim of our study was to correlate gadd45 radio- induction with the responsiveness to radiotherapy of cervical carcinomas, a type of cancer most commonly treated with radiotherapy alone. Methods: By means of a competitive polymerase chain reaction strategy, we compared in 14 patients the gene expression levels before and during external beam radiotherapy, when a dose ranging from 18 to 25 Gy was delivered to the target. Results: We found a correlation between the lack of gadd45 induction and a good clinical response to radiotherapy, in terms of both local control and disease-free survival. Conclusion: Our results support the measure of the induction of gadd45, and possibly of other genes required for regulated G1-S checkpoint, as a method useful for prognostic evaluation of cervical carcinoma patients. (C) 2000 Elsevier Science Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 411-416 |
| Number of pages | 6 |
| Journal | International Journal of Radiation Oncology Biology Physics |
| Volume | 46 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 15 2000 |
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Keywords
- Cervical carcinoma
- DNA damage checkpoints
- Gadd45
ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Radiation
Cite this
Radiation-induced gadd45 expression correlates with clinical response to radiotherapy of cervical carcinoma. / Santucci, Maria Alessandra; Barbieri, Enza; Frezza, Giovanni; Perrone, Antonio; Iacurti, Enza; Galuppi, Andrea; Salvi, Fabrizio; Bunkeila, Feisal; Neri, Stefano; Putti, Carlo; Babini, Lucio.
In: International Journal of Radiation Oncology Biology Physics, Vol. 46, No. 2, 15.01.2000, p. 411-416.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Radiation-induced gadd45 expression correlates with clinical response to radiotherapy of cervical carcinoma
AU - Santucci, Maria Alessandra
AU - Barbieri, Enza
AU - Frezza, Giovanni
AU - Perrone, Antonio
AU - Iacurti, Enza
AU - Galuppi, Andrea
AU - Salvi, Fabrizio
AU - Bunkeila, Feisal
AU - Neri, Stefano
AU - Putti, Carlo
AU - Babini, Lucio
PY - 2000/1/15
Y1 - 2000/1/15
N2 - Background: Recent work has identified a category of genes devoted to the control of genomic stability and prevention of cellular evolution. They encode components of cell cycle checkpoint, i.e., regulatory pathways committed to ordered cell cycle transition and fidelity of replicated DNA under adverse environmental conditions, such as those following exposure to genotoxic agents. Gadd45 belongs to the class II family of DNA damage- inducible (DDI) gene, and its role in DNA repair has been proved in many experimental models. The aim of our study was to correlate gadd45 radio- induction with the responsiveness to radiotherapy of cervical carcinomas, a type of cancer most commonly treated with radiotherapy alone. Methods: By means of a competitive polymerase chain reaction strategy, we compared in 14 patients the gene expression levels before and during external beam radiotherapy, when a dose ranging from 18 to 25 Gy was delivered to the target. Results: We found a correlation between the lack of gadd45 induction and a good clinical response to radiotherapy, in terms of both local control and disease-free survival. Conclusion: Our results support the measure of the induction of gadd45, and possibly of other genes required for regulated G1-S checkpoint, as a method useful for prognostic evaluation of cervical carcinoma patients. (C) 2000 Elsevier Science Inc.
AB - Background: Recent work has identified a category of genes devoted to the control of genomic stability and prevention of cellular evolution. They encode components of cell cycle checkpoint, i.e., regulatory pathways committed to ordered cell cycle transition and fidelity of replicated DNA under adverse environmental conditions, such as those following exposure to genotoxic agents. Gadd45 belongs to the class II family of DNA damage- inducible (DDI) gene, and its role in DNA repair has been proved in many experimental models. The aim of our study was to correlate gadd45 radio- induction with the responsiveness to radiotherapy of cervical carcinomas, a type of cancer most commonly treated with radiotherapy alone. Methods: By means of a competitive polymerase chain reaction strategy, we compared in 14 patients the gene expression levels before and during external beam radiotherapy, when a dose ranging from 18 to 25 Gy was delivered to the target. Results: We found a correlation between the lack of gadd45 induction and a good clinical response to radiotherapy, in terms of both local control and disease-free survival. Conclusion: Our results support the measure of the induction of gadd45, and possibly of other genes required for regulated G1-S checkpoint, as a method useful for prognostic evaluation of cervical carcinoma patients. (C) 2000 Elsevier Science Inc.
KW - Cervical carcinoma
KW - DNA damage checkpoints
KW - Gadd45
UR - http://www.scopus.com/inward/record.url?scp=0033955537&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033955537&partnerID=8YFLogxK
U2 - 10.1016/S0360-3016(99)00459-9
DO - 10.1016/S0360-3016(99)00459-9
M3 - Article
C2 - 10661348
AN - SCOPUS:0033955537
VL - 46
SP - 411
EP - 416
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
SN - 0360-3016
IS - 2
ER -