IL TEST RADIOIMMUNOLOGICO NELLA EVIDENZIAZIONE DELL'ANTIGENE AUSTRALIA (HBAG). STUDIO COMPARATIVO CON LE METODICHE DI ELETTROSINERESI E DI FISSAZIONE DEL COMPLEMENTO

Translated title of the contribution: Radioimmunoassay for detection of Australia antigen (HBAg). A comparative study with counterelectrophoresis and complement fixation test

G. Gerna, E. Magliulo, M. Barbi, E. Cattaneo

Research output: Contribution to journalArticle

Abstract

The efficiency of a solid phase radioimmunoassay in comparison with that of counterelectrophoresis and complement fixation test in the determination of HBAg was evaluated on 514 sera taken from 90 patients with acute viral hepatitis. The relative sensitivity of radioimmunoassay, as evaluated from geometric means of HBAg titers in 20 sera, appeared to be approximately 40 times higher than that of complement fixation test and about 525 times higher than that of counterelectrophoresis. Conversely, the number of patients that were positive by both complement fixation test and radioimmunoassay, was almost the same. The persistence of antigenemia at the end of hospitalization was detected in less than 10% of patients, if it was determined by counterelectrophoresis, but in about 67% of cases when determination was performed by complement fixation test and radioimmunoassay. (34 references are cited.)

Original languageItalian
Pages (from-to)1383-1389
Number of pages7
JournalGiornale di Malattie Infettive e Parassitarie
Volume26
Issue number12
Publication statusPublished - 1974

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Hepatitis B Antigens
Complement Fixation Tests
Hepatitis B Surface Antigens
Radioimmunoassay
Serum
Hepatitis
Hospitalization

ASJC Scopus subject areas

  • Parasitology

Cite this

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title = "IL TEST RADIOIMMUNOLOGICO NELLA EVIDENZIAZIONE DELL'ANTIGENE AUSTRALIA (HBAG). STUDIO COMPARATIVO CON LE METODICHE DI ELETTROSINERESI E DI FISSAZIONE DEL COMPLEMENTO",
abstract = "The efficiency of a solid phase radioimmunoassay in comparison with that of counterelectrophoresis and complement fixation test in the determination of HBAg was evaluated on 514 sera taken from 90 patients with acute viral hepatitis. The relative sensitivity of radioimmunoassay, as evaluated from geometric means of HBAg titers in 20 sera, appeared to be approximately 40 times higher than that of complement fixation test and about 525 times higher than that of counterelectrophoresis. Conversely, the number of patients that were positive by both complement fixation test and radioimmunoassay, was almost the same. The persistence of antigenemia at the end of hospitalization was detected in less than 10{\%} of patients, if it was determined by counterelectrophoresis, but in about 67{\%} of cases when determination was performed by complement fixation test and radioimmunoassay. (34 references are cited.)",
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