Radioimmunoassay of somatostatin in plasma without extraction

G. Ghirlanda, L. Uccioli, F. Perri

Research output: Contribution to journalArticle

Abstract

The assay of somatostatin (SS) in plasma shows problems related to the instability of the native peptide, the degradation of the tracer, the aspecific interactions between the plasma proteins and the different SS antisera. We have developed a simple repetitive assay in the whole plasma without extraction. Our RIA has two fundamental characteristics: 1) the antiserum, obtained in our laboratory, showed a high affinity for the antigen both in the buffer and in the plasma, 2) the careful purification of the tracer, after the iodination and just before every assay, gave us a labelled antigen with high specific activity. We have studied 40 normal subjects: SS was 104 ± 40 pg/ml. Our data were obtained in normals and in basal condition, but it is necessary to study SS variations after different stimuli to verify if this peptide has a hormonal role. Our RIA may be useful for this purpose for its sensitivity and its simplicity.

Original languageEnglish
Pages (from-to)140-141
Number of pages2
JournalIRCS Medical Science
Volume12
Issue number2
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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