Radiosensitization of human melanoma cells by ribozyme-mediated inhibition of survivin expression

Marzia Pennati, Mara Binda, Gennaro Colella, Marco Folini, Lorenzo Citti, Raffaella Villa, Maria Grazia Daidone, Nadia Zaffaroni

Research output: Contribution to journalArticle

Abstract

Survivin is a structurally unique member of the inhibitors of apoptosis protein family and is involved in the control of cell division and inhibition of apoptosis. The notion that survivin is overexpressed in most human tumors but absent in normal adult tissues with only a few exceptions has led to the proposal of survivin as a promising therapeutic target for novel anticancer therapies. In this context, we generated a hammerhead ribozyme targeting the 3′ end of the CUA110 triplet in the survivin mRNA. Two human melanoma cell lines (JR8 and M14) overexpressing survivin were stably transfected with the pRc/CMV vector carrying the ribozyme sequence. Two polyclonal cell populations proven to endogenously express ribozyme and characterized by a markedly lower survivin protein level (-60% and -50%, respectively) than JR8 and M14 parental cells were selected for the study. Ribozyme-expressing cells showed a significantly (p

Original languageEnglish
Pages (from-to)648-654
Number of pages7
JournalJournal of Investigative Dermatology
Volume120
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Keywords

  • Apoptosis
  • Ionizing radiation
  • Melanoma
  • Ribozyme
  • Survivin

ASJC Scopus subject areas

  • Dermatology

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