Abstract
Survivin is a structurally unique member of the inhibitors of apoptosis protein family and is involved in the control of cell division and inhibition of apoptosis. The notion that survivin is overexpressed in most human tumors but absent in normal adult tissues with only a few exceptions has led to the proposal of survivin as a promising therapeutic target for novel anticancer therapies. In this context, we generated a hammerhead ribozyme targeting the 3′ end of the CUA110 triplet in the survivin mRNA. Two human melanoma cell lines (JR8 and M14) overexpressing survivin were stably transfected with the pRc/CMV vector carrying the ribozyme sequence. Two polyclonal cell populations proven to endogenously express ribozyme and characterized by a markedly lower survivin protein level (-60% and -50%, respectively) than JR8 and M14 parental cells were selected for the study. Ribozyme-expressing cells showed a significantly (p
| Original language | English |
|---|---|
| Pages (from-to) | 648-654 |
| Number of pages | 7 |
| Journal | Journal of Investigative Dermatology |
| Volume | 120 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2003 |
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Keywords
- Apoptosis
- Ionizing radiation
- Melanoma
- Ribozyme
- Survivin
ASJC Scopus subject areas
- Dermatology
Cite this
Radiosensitization of human melanoma cells by ribozyme-mediated inhibition of survivin expression. / Pennati, Marzia; Binda, Mara; Colella, Gennaro; Folini, Marco; Citti, Lorenzo; Villa, Raffaella; Daidone, Maria Grazia; Zaffaroni, Nadia.
In: Journal of Investigative Dermatology, Vol. 120, No. 4, 01.04.2003, p. 648-654.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Radiosensitization of human melanoma cells by ribozyme-mediated inhibition of survivin expression
AU - Pennati, Marzia
AU - Binda, Mara
AU - Colella, Gennaro
AU - Folini, Marco
AU - Citti, Lorenzo
AU - Villa, Raffaella
AU - Daidone, Maria Grazia
AU - Zaffaroni, Nadia
PY - 2003/4/1
Y1 - 2003/4/1
N2 - Survivin is a structurally unique member of the inhibitors of apoptosis protein family and is involved in the control of cell division and inhibition of apoptosis. The notion that survivin is overexpressed in most human tumors but absent in normal adult tissues with only a few exceptions has led to the proposal of survivin as a promising therapeutic target for novel anticancer therapies. In this context, we generated a hammerhead ribozyme targeting the 3′ end of the CUA110 triplet in the survivin mRNA. Two human melanoma cell lines (JR8 and M14) overexpressing survivin were stably transfected with the pRc/CMV vector carrying the ribozyme sequence. Two polyclonal cell populations proven to endogenously express ribozyme and characterized by a markedly lower survivin protein level (-60% and -50%, respectively) than JR8 and M14 parental cells were selected for the study. Ribozyme-expressing cells showed a significantly (p
AB - Survivin is a structurally unique member of the inhibitors of apoptosis protein family and is involved in the control of cell division and inhibition of apoptosis. The notion that survivin is overexpressed in most human tumors but absent in normal adult tissues with only a few exceptions has led to the proposal of survivin as a promising therapeutic target for novel anticancer therapies. In this context, we generated a hammerhead ribozyme targeting the 3′ end of the CUA110 triplet in the survivin mRNA. Two human melanoma cell lines (JR8 and M14) overexpressing survivin were stably transfected with the pRc/CMV vector carrying the ribozyme sequence. Two polyclonal cell populations proven to endogenously express ribozyme and characterized by a markedly lower survivin protein level (-60% and -50%, respectively) than JR8 and M14 parental cells were selected for the study. Ribozyme-expressing cells showed a significantly (p
KW - Apoptosis
KW - Ionizing radiation
KW - Melanoma
KW - Ribozyme
KW - Survivin
UR - http://www.scopus.com/inward/record.url?scp=0037379377&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037379377&partnerID=8YFLogxK
U2 - 10.1046/j.1523-1747.2003.12082.x
DO - 10.1046/j.1523-1747.2003.12082.x
M3 - Article
C2 - 12648230
AN - SCOPUS:0037379377
VL - 120
SP - 648
EP - 654
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 4
ER -