Radiosynthesis and Preliminary Biological Evaluation of [18F]VC701, a Radioligand for Translocator Protein

Giuseppe Di Grigoli, Cristina Monterisi, Sara Belloli, Valeria Masiello, Letterio S alvatore Politi, Salvatore Valenti, Marco Paolino, Maurizio Anzini, Mario Matarrese, Andrea Cappelli, Rosa M aria Moresco

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Abstract

Positron emission tomography (PET) can be used to monitor in vivo translocator protein (TSPO) expression by using specific radioligands. Recently, several [11C]PK11195 analogues have been synthesized to improve binding stability and brain availability. [18F]VC701 was synthesized and validated in CD healthy rats by biodistribution and inhibition analysis. Imaging studies were also conducted on animals injected unilaterally in the striatum with quinolinic acid (QA) to evaluate the TSPO ligand uptake in a neuroinflammation/neurodegenerative model. [18F]VC701 was synthesized with a good chemical and radiochemical purity and specific activity higher than 37 GBq/μmol. Kinetic studies performed on healthy animals showed the highest tracer biodistribution in TSPO-rich organs, and preadministration of cold PK11195 caused an overall radioactivity reduction. Metabolism studies showed the absence of radiometabolites in the rat brain of QA lesioned rats, and biodistribution analysis revealed a progressive increase in radioactivity ratios (lesioned to nonlesioned striatum) during time, reaching an approximate value of 5 4 hours after tracer injection. These results encourage further evaluation of this TSPO radioligand in other models of central and peripheral diseases.

Original languageEnglish
JournalMolecular Imaging
Volume14
DOIs
Publication statusPublished - 2015

Fingerprint

Quinolinic Acid
rats
proteins
Proteins
Rats
evaluation
Radioactivity
radioactivity
brain
tracers
animals
Brain
Animals
acids
Positron emission tomography
Acids
metabolism
Metabolism
organs
Positron-Emission Tomography

ASJC Scopus subject areas

  • Biotechnology
  • Medicine(all)
  • Biomedical Engineering
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Condensed Matter Physics

Cite this

Radiosynthesis and Preliminary Biological Evaluation of [18F]VC701, a Radioligand for Translocator Protein. / Di Grigoli, Giuseppe; Monterisi, Cristina; Belloli, Sara; Masiello, Valeria; Politi, Letterio S alvatore; Valenti, Salvatore; Paolino, Marco; Anzini, Maurizio; Matarrese, Mario; Cappelli, Andrea; Moresco, Rosa M aria.

In: Molecular Imaging, Vol. 14, 2015.

Research output: Contribution to journalArticle

Di Grigoli, G, Monterisi, C, Belloli, S, Masiello, V, Politi, LSA, Valenti, S, Paolino, M, Anzini, M, Matarrese, M, Cappelli, A & Moresco, RMA 2015, 'Radiosynthesis and Preliminary Biological Evaluation of [18F]VC701, a Radioligand for Translocator Protein', Molecular Imaging, vol. 14. https://doi.org/10.2310/7290.2015.00007
Di Grigoli, Giuseppe ; Monterisi, Cristina ; Belloli, Sara ; Masiello, Valeria ; Politi, Letterio S alvatore ; Valenti, Salvatore ; Paolino, Marco ; Anzini, Maurizio ; Matarrese, Mario ; Cappelli, Andrea ; Moresco, Rosa M aria. / Radiosynthesis and Preliminary Biological Evaluation of [18F]VC701, a Radioligand for Translocator Protein. In: Molecular Imaging. 2015 ; Vol. 14.
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