Raloxifene covalently bonded to titanium implants by interfacing with (3-aminopropyl)-triethoxysilane affects osteoblast-like cell gene expression

F. Bambini, L. Greci, L. Memè, A. Santarelli, F. Carinci, F. Pezzetti, M. Procaccini, Lorenzo Lo Muzio

Research output: Contribution to journalArticle

Abstract

Since Raloxifene, a drug used in osteoporosis therapy, inhibits the osteoclast functions but not osteoblast functions, it could improve the recovery during implant surgery. This preliminary report describes a simple method to link, through a covalent bond, Raloxifene to titanium by interfacing with (3-aminopropyl)-Triethoxysilane as assessed by the IR-FT and SEM. To evaluate the biological response of osteoblast-like cells to this implant, we compared expression gene profiling of cell cultures on Raloxifene conjugated implant and normal implant by DNA microarray. By using DNA microarrays containing 19,200 genes, we identified differently expressed genes in osteoblast-like cell line (MG-63). Surface Raloxifene conjugated implants have been shown to have a relevant importance in modifying cell response. This result could be an interesting starting point for the use of an immediate functional loading of implants.

Original languageEnglish
Pages (from-to)905-914
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume19
Issue number4
Publication statusPublished - Oct 2006

Keywords

  • (3-aminopropyl)-triethoxysilane
  • cDNA microarrays
  • Covalent bond
  • Osteoblast
  • Raloxifene
  • Titanium

ASJC Scopus subject areas

  • Pharmacology

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