Ralstonia mannitolilytica infections in an oncologic day ward: Description of a cluster among high-risk patients

Claudia Lucarelli, Claudia Lucarelli, Enea Gino Di Domenico, Luigi Toma, Domenico Bracco, Grazia Prignano, Maria Fortunati, Lorella Pelagalli, Fabrizio Ensoli, Patrizio Pezzotti, Aurora García-Fernández, Annalisa Pantosti, Loredana Ingrosso, Loredana Ingrosso

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Abstract

© 2017 The Author(s). Background: Ralstonia spp, an environmental microorganism, has been occasionally associated with healthcare infections. The aim of this study was to investigate an outbreak caused by Ralstonia mannitolilytica in oncology patients. Methods: Case definition: Oncology outpatients attending a day ward, with positive blood and/or central venous catheter (CVC) culture for Ralstonia spp from September 2013 - June 2014. We analysed medical records, procedures and environmental samples. R. mannitolilytica was identified by 16S rRNA sequencing, and typed by Pulsed Field Gel Electrophoresis (PFGE); resistance to carbapenemes was investigated by phenotypic and molecular methods. Results: The patients (N=22) had different malignancies and received different therapy; all had a CVC and 16 patients presented chills and/or fever. R. mannitolilytica was isolated from both blood and CVC (n=12) or only blood (n=6) or CVC tips (n=4). The isolates had indistinguishable PFGE profile, and showed resistance to carbapenems. All the isolates were negative for carbapenemase genes while phenotypic tests suggests the presence of an AmpC β-lactamase activity,responsible for carbapenem resistance. All patients had had CVC flushed with saline to keep the venous access pervious or before receiving chemotherapy at various times before the onset of symptoms. After the first four cases occurred, the multi-dose saline bottles used for CVC flushing were replaced with single-dose vials; environmental samples were negative for R. mannitolilytica. Conclusions: Although the source of R. mannitolilytica remains unidentified, CVC flushing with contaminated saline solution seems to be the most likely origin of R. mannitolilytica CVC colonization and subsequent infections. In order to prevent similar outbreaks we recommend removal of any CVC that is no longer necessary and the use of single-dose solutions for any parenteral treatment of oncology patients.
Original languageEnglish
JournalAntimicrobial Resistance and Infection Control
Volume6
Issue number1
DOIs
Publication statusPublished - Feb 7 2017

Keywords

  • Carbapenem resistance
  • Central venous catheter
  • Oncologic patients
  • Outbreak
  • Ralstonia mannitolilytica

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    Lucarelli, C., Lucarelli, C., Di Domenico, E. G., Toma, L., Bracco, D., Prignano, G., Fortunati, M., Pelagalli, L., Ensoli, F., Pezzotti, P., García-Fernández, A., Pantosti, A., Ingrosso, L., & Ingrosso, L. (2017). Ralstonia mannitolilytica infections in an oncologic day ward: Description of a cluster among high-risk patients. Antimicrobial Resistance and Infection Control, 6(1). https://doi.org/10.1186/s13756-017-0178-z