Raman and SERS recognition of β-carotene and haemoglobin fingerprints in human whole blood

Michele Casella, Andrea Lucotti, Matteo Tommasini, Marzia Bedoni, Elena Forvi, Furio Gramatica, Giuseppe Zerbi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The present work reports on Raman and Surface Enhanced Raman Scattering (SERS) vibrational fingerprints of β-carotene and haemoglobin in fresh whole blood (i.e. right after blood test) with different laser excitations, i.e. visible (514 nm) and near-infrared (NIR, 785 nm). The use of colloidal silver nanoparticles significantly increases the Raman signal, thus providing a clear SERS spectrum of blood. The collected spectra have been examined and marker bands of β-carotene and of the haem prosthetic group of haemoglobin have been found. In particular, the fundamental features of β-carotene (514 nm excitation), blood proteins and haem molecules (785 nm excitation) were recognized and assigned. Moreover haemoglobin SERS signals can be identified and related with its oxygenation state (oxy-haemoglobin). The data reported show the prospects of Raman and SERS techniques to detect important bio-molecules in a whole blood sample with no pre-treatment.

Original languageEnglish
Pages (from-to)915-919
Number of pages5
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume79
Issue number5
DOIs
Publication statusPublished - Sep 2011

Fingerprint

carotene
Hemoglobin
hemoglobin
Carotenoids
blood
Raman scattering
Hemoglobins
Blood
Raman spectra
Heme
Molecules
Laser excitation
Oxygenation
excitation
oxyhemoglobin
Prosthetics
oxygenation
Blood Proteins
pretreatment
markers

Keywords

  • β-Carotene
  • Haemoglobin
  • Raman spectroscopy
  • Surface Enhanced Raman Scattering (SERS)
  • Whole blood

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Spectroscopy

Cite this

Raman and SERS recognition of β-carotene and haemoglobin fingerprints in human whole blood. / Casella, Michele; Lucotti, Andrea; Tommasini, Matteo; Bedoni, Marzia; Forvi, Elena; Gramatica, Furio; Zerbi, Giuseppe.

In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 79, No. 5, 09.2011, p. 915-919.

Research output: Contribution to journalArticle

Casella, Michele ; Lucotti, Andrea ; Tommasini, Matteo ; Bedoni, Marzia ; Forvi, Elena ; Gramatica, Furio ; Zerbi, Giuseppe. / Raman and SERS recognition of β-carotene and haemoglobin fingerprints in human whole blood. In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2011 ; Vol. 79, No. 5. pp. 915-919.
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