Raman spectroscopy and imaging: Promising optical diagnostic tools in pediatrics

C. Beleites, A. Bonifacio, D. Codrich, C. Krafft, V. Sergo

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This review focuses on the use of Raman spectroscopy, an analytical technique based on the inelastic scattering of harmless laser light with biological tissues, as an innovative diagnostic tool in pediatrics. After a brief introduction to explain the fundamental concepts behind Raman spectroscopy and imaging, a short summary is given of the most important and common issues arising when handling spectral data with multivariate statistics. Then, the most relevant papers in which Raman spectroscopy or imaging has been applied with diagnostic purposes to pediatric patients are reviewed, and grouped according to the type of pathology: neoplastic, inflammatory, allergic, malformative as well as other kinds. Raman spectroscopy has been used both in vivo, mostly using optical fibers for tissue illumination, as well as on ex vivo tissue sections in a microscopic imaging approach defined as 'spectral histopathology'. According to the results reported so far, this technique showed a huge potential for mini- or non-invasive real-time, bedside and intra-operatory diagnosis, as well as for an ex vivo imaging tool in support to pathologists. Despite many studies are limited by the small sample size, this technique is extremely promising in terms of sensitivity and specificity.

Original languageEnglish
Pages (from-to)2176-2187
Number of pages12
JournalCurrent Medicinal Chemistry
Volume20
Issue number17
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Pediatrics
Raman Spectrum Analysis
Optical Imaging
Raman spectroscopy
Imaging techniques
Tissue
Optical Fibers
Inelastic scattering
Pathology
Lighting
Sample Size
Optical fibers
Lasers
Statistics
Light
Sensitivity and Specificity

Keywords

  • Diagnosis
  • Imaging
  • Mapping
  • Raman
  • Spectroscopy

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Raman spectroscopy and imaging : Promising optical diagnostic tools in pediatrics. / Beleites, C.; Bonifacio, A.; Codrich, D.; Krafft, C.; Sergo, V.

In: Current Medicinal Chemistry, Vol. 20, No. 17, 06.2013, p. 2176-2187.

Research output: Contribution to journalArticle

Beleites, C. ; Bonifacio, A. ; Codrich, D. ; Krafft, C. ; Sergo, V. / Raman spectroscopy and imaging : Promising optical diagnostic tools in pediatrics. In: Current Medicinal Chemistry. 2013 ; Vol. 20, No. 17. pp. 2176-2187.
@article{472651da4ff34078b27070dfe4eac10a,
title = "Raman spectroscopy and imaging: Promising optical diagnostic tools in pediatrics",
abstract = "This review focuses on the use of Raman spectroscopy, an analytical technique based on the inelastic scattering of harmless laser light with biological tissues, as an innovative diagnostic tool in pediatrics. After a brief introduction to explain the fundamental concepts behind Raman spectroscopy and imaging, a short summary is given of the most important and common issues arising when handling spectral data with multivariate statistics. Then, the most relevant papers in which Raman spectroscopy or imaging has been applied with diagnostic purposes to pediatric patients are reviewed, and grouped according to the type of pathology: neoplastic, inflammatory, allergic, malformative as well as other kinds. Raman spectroscopy has been used both in vivo, mostly using optical fibers for tissue illumination, as well as on ex vivo tissue sections in a microscopic imaging approach defined as 'spectral histopathology'. According to the results reported so far, this technique showed a huge potential for mini- or non-invasive real-time, bedside and intra-operatory diagnosis, as well as for an ex vivo imaging tool in support to pathologists. Despite many studies are limited by the small sample size, this technique is extremely promising in terms of sensitivity and specificity.",
keywords = "Diagnosis, Imaging, Mapping, Raman, Spectroscopy",
author = "C. Beleites and A. Bonifacio and D. Codrich and C. Krafft and V. Sergo",
year = "2013",
month = "6",
doi = "10.2174/0929867311320170003",
language = "English",
volume = "20",
pages = "2176--2187",
journal = "Current Medicinal Chemistry",
issn = "0929-8673",
publisher = "Bentham Science Publishers B.V.",
number = "17",

}

TY - JOUR

T1 - Raman spectroscopy and imaging

T2 - Promising optical diagnostic tools in pediatrics

AU - Beleites, C.

AU - Bonifacio, A.

AU - Codrich, D.

AU - Krafft, C.

AU - Sergo, V.

PY - 2013/6

Y1 - 2013/6

N2 - This review focuses on the use of Raman spectroscopy, an analytical technique based on the inelastic scattering of harmless laser light with biological tissues, as an innovative diagnostic tool in pediatrics. After a brief introduction to explain the fundamental concepts behind Raman spectroscopy and imaging, a short summary is given of the most important and common issues arising when handling spectral data with multivariate statistics. Then, the most relevant papers in which Raman spectroscopy or imaging has been applied with diagnostic purposes to pediatric patients are reviewed, and grouped according to the type of pathology: neoplastic, inflammatory, allergic, malformative as well as other kinds. Raman spectroscopy has been used both in vivo, mostly using optical fibers for tissue illumination, as well as on ex vivo tissue sections in a microscopic imaging approach defined as 'spectral histopathology'. According to the results reported so far, this technique showed a huge potential for mini- or non-invasive real-time, bedside and intra-operatory diagnosis, as well as for an ex vivo imaging tool in support to pathologists. Despite many studies are limited by the small sample size, this technique is extremely promising in terms of sensitivity and specificity.

AB - This review focuses on the use of Raman spectroscopy, an analytical technique based on the inelastic scattering of harmless laser light with biological tissues, as an innovative diagnostic tool in pediatrics. After a brief introduction to explain the fundamental concepts behind Raman spectroscopy and imaging, a short summary is given of the most important and common issues arising when handling spectral data with multivariate statistics. Then, the most relevant papers in which Raman spectroscopy or imaging has been applied with diagnostic purposes to pediatric patients are reviewed, and grouped according to the type of pathology: neoplastic, inflammatory, allergic, malformative as well as other kinds. Raman spectroscopy has been used both in vivo, mostly using optical fibers for tissue illumination, as well as on ex vivo tissue sections in a microscopic imaging approach defined as 'spectral histopathology'. According to the results reported so far, this technique showed a huge potential for mini- or non-invasive real-time, bedside and intra-operatory diagnosis, as well as for an ex vivo imaging tool in support to pathologists. Despite many studies are limited by the small sample size, this technique is extremely promising in terms of sensitivity and specificity.

KW - Diagnosis

KW - Imaging

KW - Mapping

KW - Raman

KW - Spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84877966079&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84877966079&partnerID=8YFLogxK

U2 - 10.2174/0929867311320170003

DO - 10.2174/0929867311320170003

M3 - Article

C2 - 23458614

AN - SCOPUS:84877966079

VL - 20

SP - 2176

EP - 2187

JO - Current Medicinal Chemistry

JF - Current Medicinal Chemistry

SN - 0929-8673

IS - 17

ER -