Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells

A Gualerzi, S Niada, Chiara Giannasi, Silvia Picciolini, C Morasso, R Vanna, V Rossella, M Masserini, M Bedoni, F Ciceri, ME Bernardo, AT Brini, F Gramatica

Research output: Contribution to journalArticle

Abstract

Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. © 2017 The Author(s).
Original languageEnglish
Article number9820
JournalScientific Reports
Volume7
Issue number26
DOIs
Publication statusPublished - 2017

Fingerprint

Raman Spectrum Analysis
Mesenchymal Stromal Cells
Immunomodulation
Sphingomyelins
Gangliosides
Dermatoglyphics
Extracellular Vesicles
Adipose Tissue
Regeneration
Fibroblasts
Bone Marrow
Bone and Bones
Skin
Research

Cite this

Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells. / Gualerzi, A; Niada, S; Giannasi, Chiara; Picciolini, Silvia; Morasso, C; Vanna, R; Rossella, V; Masserini, M; Bedoni, M; Ciceri, F; Bernardo, ME; Brini, AT; Gramatica, F.

In: Scientific Reports, Vol. 7, No. 26, 9820, 2017.

Research output: Contribution to journalArticle

@article{5a4c504d74f24a07beca966f1902dd08,
title = "Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells",
abstract = "Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7{\%}. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. {\circledC} 2017 The Author(s).",
author = "A Gualerzi and S Niada and Chiara Giannasi and Silvia Picciolini and C Morasso and R Vanna and V Rossella and M Masserini and M Bedoni and F Ciceri and ME Bernardo and AT Brini and F Gramatica",
year = "2017",
doi = "10.1038/s41598-017-10448-1",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "26",

}

TY - JOUR

T1 - Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells

AU - Gualerzi, A

AU - Niada, S

AU - Giannasi, Chiara

AU - Picciolini, Silvia

AU - Morasso, C

AU - Vanna, R

AU - Rossella, V

AU - Masserini, M

AU - Bedoni, M

AU - Ciceri, F

AU - Bernardo, ME

AU - Brini, AT

AU - Gramatica, F

PY - 2017

Y1 - 2017

N2 - Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. © 2017 The Author(s).

AB - Extracellular vesicles (EVs) from mesenchymal stromal cells (MSC) are emerging as valuable therapeutic agents for tissue regeneration and immunomodulation, but their clinical applications have so far been limited by the technical restraints of current isolation and characterisation procedures. This study shows for the first time the successful application of Raman spectroscopy as label-free, sensitive and reproducible means of carrying out the routine bulk characterisation of MSC-derived vesicles before their use in vitro or in vivo, thus promoting the translation of EV research to clinical practice. The Raman spectra of the EVs of bone marrow and adipose tissue-derived MSCs were compared with human dermal fibroblast EVs in order to demonstrate the ability of the method to distinguish the vesicles of the three cytotypes automatically with an accuracy of 93.7%. Our data attribute a Raman fingerprint to EVs from undifferentiated and differentiated cells of diverse tissue origin, and provide insights into the biochemical characteristics of EVs from different sources and into the differential contribution of sphingomyelin, gangliosides and phosphatidilcholine to the Raman spectra themselves. © 2017 The Author(s).

U2 - 10.1038/s41598-017-10448-1

DO - 10.1038/s41598-017-10448-1

M3 - Article

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 26

M1 - 9820

ER -