RANTES and MIP-1α production by T lymphocytes, monocytes and NK cells from nonagenarian subjects

Erminia Mariani, Lia Pulsatelli, Simona Neri, Paolo Dolzani, Alessandra Meneghetti, Tania Silvestri, Giovanni Ravaglia, Paola Forti, Luca Cattini, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

While numerous previous studies have investigated age-related changes of cytokine production, little is known about chemokines, the importance of which in regulating immune response is becoming increasingly evident. In this study, a group of healthy subjects over 90 years old is compared to a group of young subjects, we evaluated the ability of monocytes, T lymphocytes and NK cells: (1) to produce RANTES and MIP-1α, either in basal conditions or after stimulation with, respectively, LPS, anti-CD3 MoAb and IL-2; (2) to express the corresponding chemokine receptors (CCR1, CCR3, CCR5). We demonstrate that: (a) monocytes, T lymphocytes and NK cells spontaneously produced detectable amounts of chemokines, both in young and old subjects; (b) monocyte-dependent RANTES and MIP-1α production induced by LPS was up-regulated in nonagenarian subjects as anti-CD3-induced secretion from T cells; (c) RANTES and MIP-1α production by IL-2 stimulated NK cells was reduced in elderly subjects; (d) CCR1, CCR3 and CCR5 were widely expressed on monocytes, but less expressed on T lymphocytes and NK cells. The diversity within PBMC might reflect their different states of activation and/or responsiveness, influencing the ability to develop rapid innate and long-lasting adaptive immune responses.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalExperimental Gerontology
Volume37
Issue number2-3
DOIs
Publication statusPublished - Jan 3 2002

Keywords

  • Ageing
  • MIP-1α
  • Monocyte
  • NK cell
  • RANTES
  • T lymphocyte

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

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