RANTES and MIP-1α production by T lymphocytes, monocytes and NK cells from nonagenarian subjects

Erminia Mariani, Lia Pulsatelli, Simona Neri, Paolo Dolzani, Alessandra Meneghetti, Tania Silvestri, Giovanni Ravaglia, Paola Forti, Luca Cattini, Andrea Facchini

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

While numerous previous studies have investigated age-related changes of cytokine production, little is known about chemokines, the importance of which in regulating immune response is becoming increasingly evident. In this study, a group of healthy subjects over 90 years old is compared to a group of young subjects, we evaluated the ability of monocytes, T lymphocytes and NK cells: (1) to produce RANTES and MIP-1α, either in basal conditions or after stimulation with, respectively, LPS, anti-CD3 MoAb and IL-2; (2) to express the corresponding chemokine receptors (CCR1, CCR3, CCR5). We demonstrate that: (a) monocytes, T lymphocytes and NK cells spontaneously produced detectable amounts of chemokines, both in young and old subjects; (b) monocyte-dependent RANTES and MIP-1α production induced by LPS was up-regulated in nonagenarian subjects as anti-CD3-induced secretion from T cells; (c) RANTES and MIP-1α production by IL-2 stimulated NK cells was reduced in elderly subjects; (d) CCR1, CCR3 and CCR5 were widely expressed on monocytes, but less expressed on T lymphocytes and NK cells. The diversity within PBMC might reflect their different states of activation and/or responsiveness, influencing the ability to develop rapid innate and long-lasting adaptive immune responses.

Original languageEnglish
Pages (from-to)219-226
Number of pages8
JournalExperimental Gerontology
Volume37
Issue number2-3
DOIs
Publication statusPublished - Jan 3 2002

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Chemokine CCL5
T-cells
Natural Killer Cells
Monocytes
T-Lymphocytes
Chemokines
Interleukin-2
Chemokine Receptors
Adaptive Immunity
Healthy Volunteers
Chemical activation
Cytokines

Keywords

  • Ageing
  • MIP-1α
  • Monocyte
  • NK cell
  • RANTES
  • T lymphocyte

ASJC Scopus subject areas

  • Ageing
  • Medicine(all)

Cite this

RANTES and MIP-1α production by T lymphocytes, monocytes and NK cells from nonagenarian subjects. / Mariani, Erminia; Pulsatelli, Lia; Neri, Simona; Dolzani, Paolo; Meneghetti, Alessandra; Silvestri, Tania; Ravaglia, Giovanni; Forti, Paola; Cattini, Luca; Facchini, Andrea.

In: Experimental Gerontology, Vol. 37, No. 2-3, 03.01.2002, p. 219-226.

Research output: Contribution to journalArticle

Mariani, Erminia ; Pulsatelli, Lia ; Neri, Simona ; Dolzani, Paolo ; Meneghetti, Alessandra ; Silvestri, Tania ; Ravaglia, Giovanni ; Forti, Paola ; Cattini, Luca ; Facchini, Andrea. / RANTES and MIP-1α production by T lymphocytes, monocytes and NK cells from nonagenarian subjects. In: Experimental Gerontology. 2002 ; Vol. 37, No. 2-3. pp. 219-226.
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