Rapamycin treatment of Mandibuloacral Dysplasia cells rescues localization of chromatin-associated proteins and cell cycle dynamics

Vittoria Cenni, Cristina Capanni, Elisabetta Mattioli, Marta Columbaro, Manfred Wehnert, Michela Ortolani, Milena Fini, Giuseppe Novelli, Jessika Bertacchini, Nadir M. Maraldi, Sandra Marmiroli, Maria Rosaria D'Apice, Sabino Prencipe, Stefano Squarzoni, Giovanna Lattanzi

Research output: Contribution to journalArticlepeer-review

Abstract

Lamin A is a key component of the nuclear lamina produced through post-translational processing of its precursor known as prelamin A. LMNA mutations leading to farnesylated prelamin A accumulation are known to cause lipodystrophy, progeroid and developmental diseases, including Mandibuloacral dysplasia, a mild progeroid syndrome with partial lipodystrophy and altered bone turnover. Thus, degradation of prelamin A is expected to improve the disease phenotype. Here, we show different susceptibilities of prelamin A forms to proteolysis and further demonstrate that treatment with rapamycin efficiently and selectively triggers lysosomal degradation of farnesylated prelamin A, the most toxic processing intermediate. Importantly, rapamycin treatment of Mandibuloacral dysplasia cells, which feature very low levels of the NAD-dependent sirtuin SIRT-1 in the nuclear matrix, restores SIRT-1 localization and distribution of chromatin markers, elicits release of the transcription factor Oct-1 and determines shortening of the prolonged S-phase. These findings indicate the drug as a possible treatment for Mandibuloacral dysplasia.

Original languageEnglish
Pages (from-to)755-770
Number of pages16
JournalAging
Volume6
Issue number9
Publication statusPublished - 2014

Keywords

  • Mandibuloacral Dysplasia (MADA)
  • Oct-1
  • Prelamin A
  • Rapamycin
  • SIRT-1

ASJC Scopus subject areas

  • Ageing
  • Cell Biology
  • Medicine(all)

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