Rapid and efficient magnetization of mesenchymal stem cells by dendrimer-functionalized magnetic nanoparticles

Nunzia Di Maggio, Elisa Martella, Steve Meikle, Marta Columbaro, Enrico Lucarelli, Matteo Santin, Andrea Banfi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: Rapid and efficient magnetization of human bone marrow stromal cells (BMSC) through functionalized magnetic nanoparticles (MNP). Methods: MNP were functionalized with poly(epsilon-lysine) dendrons exposing carboxybetaine residue (CB-MNP) to enhance binding to the cellular glycocalix. BMSC were incubated with CB-MNP or non-functionalized PAA-MNP for 5-30 min in suspension. Results: CB-MNP functionalization increased the magnetization efficiency by threefold. Remarkably, 66% of cells were magnetized after only 5 min and the maximum efficiency of >80% was reached by 15 min. BMSC viability, proliferation and differentiation were not impaired: actually, adipogenic and osteogenic differentiation were even improved. Conclusion: Carboxybetaine-dendron functionalization ensured rapid and efficient BMSC magnetization and allowed innovative suspension labeling, with a potential for bypassing adhesion culture of progenitors for regenerative medicine.

Original languageEnglish
Pages (from-to)1519-1534
Number of pages16
JournalNanomedicine
Volume11
Issue number12
DOIs
Publication statusPublished - Jun 1 2016

Fingerprint

Dendrimers
Stem cells
Mesenchymal Stromal Cells
magnetization
Nanoparticles
Magnetization
stem
efficiency
bone
Bone
proliferation
medicine
Suspensions
magnetic method
Glycocalyx
Regenerative Medicine
Cell proliferation
adhesion
Labeling
Lysine

Keywords

  • adipogenesis
  • carboxybetaine
  • dendrimers
  • magnetization
  • nanoparticles
  • osteogenesis
  • stem cells
  • suspension culture

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Rapid and efficient magnetization of mesenchymal stem cells by dendrimer-functionalized magnetic nanoparticles. / Di Maggio, Nunzia; Martella, Elisa; Meikle, Steve; Columbaro, Marta; Lucarelli, Enrico; Santin, Matteo; Banfi, Andrea.

In: Nanomedicine, Vol. 11, No. 12, 01.06.2016, p. 1519-1534.

Research output: Contribution to journalArticle

Di Maggio, Nunzia ; Martella, Elisa ; Meikle, Steve ; Columbaro, Marta ; Lucarelli, Enrico ; Santin, Matteo ; Banfi, Andrea. / Rapid and efficient magnetization of mesenchymal stem cells by dendrimer-functionalized magnetic nanoparticles. In: Nanomedicine. 2016 ; Vol. 11, No. 12. pp. 1519-1534.
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AU - Meikle, Steve

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AU - Lucarelli, Enrico

AU - Santin, Matteo

AU - Banfi, Andrea

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