Rapid autofocusing for dynamic observation exploiting holographic polarization microscope

Leiping Che, Wen Xiao, Pietro Ferraro, Feng Pan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We proposed a rapid autofocusing method exploiting holographic polarization microscope, which could determine the refocusing distance without multiple reconstructions and complex network training. The defocus distance of the object is calculated rapidly by identifying the separation distance of the two defocus images of the object and using the linear relation between the defocus distance of the object and the separation distance of the two defocus images of the object. The linear relationship is obtained by numerical propagation and linear fitting. Last, we have demonstrated the effectiveness of the proposed method by reconstructing the in-focus images of flowing cells. We believe that our proposed method could be a powerful tool for dynamic observation and extend the application of holographic polarization microscope.

Original languageEnglish
Title of host publicationOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V
EditorsPietro Ferraro, Simonetta Grilli, Monika Ritsch-Marte, Christoph K. Hitzenberger
PublisherSPIE
ISBN (Electronic)9781510644069
DOIs
Publication statusPublished - 2021
EventOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021 - Virtual, Online, Germany
Duration: Jun 21 2021Jun 25 2021

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11786
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceOptical Methods for Inspection, Characterization, and Imaging of Biomaterials V 2021
Country/TerritoryGermany
CityVirtual, Online
Period6/21/216/25/21

Keywords

  • Autofocusing
  • Holographic microscopy
  • Holographic polarization microscopy
  • Off-axis illumination

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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