Abstract
A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.
Original language | English |
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Pages (from-to) | 879-884 |
Number of pages | 6 |
Journal | Biotechnology Techniques |
Volume | 9 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 1995 |
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry