Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

Luca Del Giacco, Luisa Doneda, Paola Grammatico, Lidia Larizza

Research output: Contribution to journalArticlepeer-review

Abstract

A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.

Original languageEnglish
Pages (from-to)879-884
Number of pages6
JournalBiotechnology Techniques
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 1995

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

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