Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

Luca Del Giacco; Luisa Doneda; Paola Grammatico; Lidia Larizza

doi:10.1007/BF00158540

Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

Luca Del Giacco, Luisa Doneda, Paola Grammatico, Lidia Larizza

Fondazione Istituto Auxologico Italiano

Research output: Contribution to journal › Article › peer-review

Abstract

A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.

Original language	English
Pages (from-to)	879-884
Number of pages	6
Journal	Biotechnology Techniques
Volume	9
Issue number	12
DOIs	https://doi.org/10.1007/BF00158540
Publication status	Published - Dec 1995

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Biochemistry

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title = "Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.",

abstract = "A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.",

author = "{Del Giacco}, Luca and Luisa Doneda and Paola Grammatico and Lidia Larizza",

year = "1995",

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doi = "10.1007/BF00158540",

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AU - Doneda, Luisa

AU - Grammatico, Paola

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PY - 1995/12

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AB - A novel approach of competitive PCR for quantifying mRNAs is described where competitors are constructed by deleting an internal sequence from target reverse transcribed mRNAs. The two sets of product generated (control and target) are easily discriminated by size and quantified for the sequence of interest and a housekeeping gene, β-actin,used as internal mRNA control.

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Rapid construction of competitive templates for quantitative PCR of reverse-transcribed mRNAs.

Abstract

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