Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction

E. Zino, S. Di Terlizzi, C. Carugo, L. Baggi, A. Galli, C. Bonini, S. Rossini, B. Mazzi, K. Fleischhauer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Human leucocyte antigen-B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence-specific primer-based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence-specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalTissue Antigens
Volume63
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

HLA-B Antigens
Polymerase chain reaction
Alleles
Polymerase Chain Reaction
HLA Antigens
Amplification
Reactive Arthritis
Screening
Ankylosing Spondylitis
Rheumatic Diseases
Research

Keywords

  • Diagnosis of rheumatic diseases
  • Group-specific detection
  • HLA-B*27
  • Sequence-specific primer

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction. / Zino, E.; Di Terlizzi, S.; Carugo, C.; Baggi, L.; Galli, A.; Bonini, C.; Rossini, S.; Mazzi, B.; Fleischhauer, K.

In: Tissue Antigens, Vol. 63, No. 1, 01.2004, p. 88-92.

Research output: Contribution to journalArticle

Zino, E, Di Terlizzi, S, Carugo, C, Baggi, L, Galli, A, Bonini, C, Rossini, S, Mazzi, B & Fleischhauer, K 2004, 'Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction', Tissue Antigens, vol. 63, no. 1, pp. 88-92. https://doi.org/10.1111/j.1399-0039.2004.00158.x
Zino, E. ; Di Terlizzi, S. ; Carugo, C. ; Baggi, L. ; Galli, A. ; Bonini, C. ; Rossini, S. ; Mazzi, B. ; Fleischhauer, K. / Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction. In: Tissue Antigens. 2004 ; Vol. 63, No. 1. pp. 88-92.
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