Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction

E. Zino, S. Di Terlizzi, C. Carugo, L. Baggi, A. Galli, C. Bonini, S. Rossini, B. Mazzi, K. Fleischhauer

Research output: Contribution to journalArticle

Abstract

Human leucocyte antigen-B*27 is strongly associated with a number of rheumatic diseases, including ankylosing spondylitis and reactive arthritis. Targeted detection of the B*27 group by molecular methods is hampered by the extreme heterogeneity of the serological B*27 group. Here, we describe a simple, rapid sequence-specific primer-based method for detection of all 28 B*27 alleles defined to date. The method involves an initial screening with two sequence-specific polymerase chain reactions (PCRs), which has to be followed by two additional PCR amplifications in samples carrying a few rare subtypes of B*27, B*4202 or B*7301. The described protocol should be useful for laboratories involved in diagnostics and research of rheumatoid diseases.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalTissue Antigens
Volume63
Issue number1
DOIs
Publication statusPublished - Jan 2004

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Keywords

  • Diagnosis of rheumatic diseases
  • Group-specific detection
  • HLA-B*27
  • Sequence-specific primer

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Zino, E., Di Terlizzi, S., Carugo, C., Baggi, L., Galli, A., Bonini, C., Rossini, S., Mazzi, B., & Fleischhauer, K. (2004). Rapid detection of all HLA-B*27 alleles (B*2701-B*2725) by group-specific polymerase chain reaction. Tissue Antigens, 63(1), 88-92. https://doi.org/10.1111/j.1399-0039.2004.00158.x