TY - JOUR
T1 - Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies
AU - Percivalle, Elena
AU - Sarasini, Antonella
AU - Visai, Livia
AU - Revello, M. Grazia
AU - Gerna, Giuseppe
PY - 2005/7
Y1 - 2005/7
N2 - Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.
AB - Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.
UR - http://www.scopus.com/inward/record.url?scp=22144442111&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22144442111&partnerID=8YFLogxK
U2 - 10.1128/JCM.43.7.3443-3446.2005
DO - 10.1128/JCM.43.7.3443-3446.2005
M3 - Article
C2 - 16000473
AN - SCOPUS:22144442111
VL - 43
SP - 3443
EP - 3446
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 7
ER -