Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies

Elena Percivalle, Antonella Sarasini, Livia Visai, M. Grazia Revello, Giuseppe Gerna

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.

Original languageEnglish
Pages (from-to)3443-3446
Number of pages4
JournalJournal of Clinical Microbiology
Volume43
Issue number7
DOIs
Publication statusPublished - Jul 2005

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies'. Together they form a unique fingerprint.

Cite this