Abstract
Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert®. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert® assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 323-326 |
| Number of pages | 4 |
| Journal | Leukemia Research |
| Volume | 32 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2008 |
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Keywords
- Cell lines
- Hematopoietic cell lines
- Microbiological agar culture
- MycoAlert
- Mycoplasma
- PCR-ELISA assay
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test. / Mariotti, E.; Mirabelli, P.; Di Noto, R.; Fortunato, G.; Salvatore, F.
In: Leukemia Research, Vol. 32, No. 2, 02.2008, p. 323-326.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test
AU - Mariotti, E.
AU - Mirabelli, P.
AU - Di Noto, R.
AU - Fortunato, G.
AU - Salvatore, F.
PY - 2008/2
Y1 - 2008/2
N2 - Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert®. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert® assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.
AB - Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert®. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert® assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.
KW - Cell lines
KW - Hematopoietic cell lines
KW - Microbiological agar culture
KW - MycoAlert
KW - Mycoplasma
KW - PCR-ELISA assay
UR - http://www.scopus.com/inward/record.url?scp=38949092488&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38949092488&partnerID=8YFLogxK
U2 - 10.1016/j.leukres.2007.04.010
DO - 10.1016/j.leukres.2007.04.010
M3 - Article
VL - 32
SP - 323
EP - 326
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 2
ER -