Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test

E. Mariotti; P. Mirabelli; R. Di Noto; G. Fortunato; F. Salvatore

doi:10.1016/j.leukres.2007.04.010

Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test

E. Mariotti, P. Mirabelli, R. Di Noto, G. Fortunato, F. Salvatore

IRCCS SDN

Research output: Contribution to journal › Article › peer-review

Abstract

Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert^®. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert^® assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.

Original language	English
Pages (from-to)	323-326
Number of pages	4
Journal	Leukemia Research
Volume	32
Issue number	2
DOIs	https://doi.org/10.1016/j.leukres.2007.04.010
Publication status	Published - Feb 2008

Keywords

Cell lines
Hematopoietic cell lines
Microbiological agar culture
MycoAlert
Mycoplasma
PCR-ELISA assay

ASJC Scopus subject areas

Cancer Research
Hematology
Oncology

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title = "Rapid detection of mycoplasma in continuous cell lines using a selective biochemical test",

abstract = "Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert{\textregistered}. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert{\textregistered} assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.",

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AU - Mariotti, E.

AU - Mirabelli, P.

AU - Di Noto, R.

AU - Fortunato, G.

AU - Salvatore, F.

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AB - Mycoplasma contamination is a deleterious event for a cell culture laboratory due to the ability of this microorganism to contaminate cell culture leading up to the production of false data or, in the worst cases, to the loss of cell culture itself. Fortunately, mycoplasma can be eradicated by the use of antibiotics, but early detection of contamination is peremptory. Here, we propose the use of a sensitive and specific biochemical test named MycoAlert®. In particular, as regards cell cultures not yet treated with antibiotics, sensitivity, specificity, positive and negative predictive values of MycoAlert® assay gave excellent scores of 100%, 97%, 89% and 100%, respectively.

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