Rapid, differential diagnosis of orthopox- and herpesviruses based upon real-time PCR product melting temperature and restriction enzyme analysis of amplicons

Fabrizio Carletti, Antonino Di Caro, Silvia Calcaterra, Allen Grolla, Markus Czub, Giuseppe Ippolito, Maria R. Capobianchi, Douglas Horejsh

Research output: Contribution to journalArticle

Abstract

Orthopoxviruses tend to have non-specific early symptoms that cannot be differentiated readily from other infectious exanthemas, such as varicella-zoster virus (VZV) or disseminated herpes simplex virus (HSV) infections. A rapid assay was developed for the differential diagnosis of orthopoxviruses and herpesviruses based upon the melting temperatures of real-time PCR amplicons. A mean melting temperature difference of 8.7°C was observed between the products amplified from the two virus families. Further identification of individual pathogens was made using restriction enzyme analysis. The assay was able to identify correctly viruses from quality control panels of herpes and orthopoxviruses.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalJournal of Virological Methods
Volume129
Issue number1
DOIs
Publication statusPublished - Oct 2005

ASJC Scopus subject areas

  • Virology

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