Rapid drug susceptibility of Mycobacterium avium complex using a fluorescence quenching method

P. Marone, L. Bono, E. Carretto, D. Barbarini, S. Telecco

Research output: Contribution to journalArticlepeer-review

Abstract

Mycobacteria Growth Indicator Tube (MGIT) is a recently introduced rapid growth detection method which uses an oxygen quenched fluorescent indicator. The present study evaluated the ability of this new method to determine the drug susceptibility of Mycobacterium avium complex (MAC). Thirty strains recovered from patients with AIDS were tested for susceptibility to clarithromycin, rifabutin, ciprofloxacin, azithromycin and amikacin using MGIT. Results were compared to susceptibilities determined by the agar dilution method. The results obtained showed a 100% correlation between MGIT and the agar dilution method for rifabutin and clarithromycin. There was a 100% correlation between the two methods for azithromycin against 27 strains. MGIT was well correlated with the agar dilution method for detecting resistance to clarithromycin, rifabutin and azithromycin in 4 days, but the correlation was poor when susceptibilities to ciprofloxacin and amikacin were determined. This rapid method is non-radiometric, noninvasive and does not require any special instruments.

Original languageEnglish
Pages (from-to)247-250
Number of pages4
JournalJournal of Chemotherapy
Volume9
Issue number4
Publication statusPublished - 1997

Keywords

  • Agar dilution method
  • Antimicrobial susceptibility test
  • Fluorescence quenching method
  • Mycobacterium avium complex

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Microbiology (medical)

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