Rapid identification of Mycobacterium tuberculosis and Mycobacterium avium by polymerase chain reaction and restriction enzyme analysis within sigma factor regions

S. Comincini, D. Barbarini, S. Telecco, L. Bono, P. Marone

Research output: Contribution to journal › Article › peer-review

Abstract

A single Polymerase Chain Reaction (PCR) within the rpoV gene was developed to rapidly distinguish mycobacteria isolated from clinical specimens. The species identifications of Mycobacterium avium complex (MAC) and Mycobacterium tuberculosis were congruent with standard typing techniques. The analysis was targeted toward the identification of species-specific markers for the clinically relevant M. tuberculosis and M. avium. In addition, HaeIII digestion of the amplification products yielded isolates-specific bands.

Original languageEnglish
Pages (from-to)391-395
Number of pages5
JournalNew Microbiologica
Volume21
Issue number4
Publication statusPublished - Oct 1998

Keywords

  • Mycobacteria identification
  • Mycobacterium avium
  • Mycobacterium tuberculosis
  • rpoV gene
  • Sigma factor

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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