Abstract
A single Polymerase Chain Reaction (PCR) within the rpoV gene was developed to rapidly distinguish mycobacteria isolated from clinical specimens. The species identifications of Mycobacterium avium complex (MAC) and Mycobacterium tuberculosis were congruent with standard typing techniques. The analysis was targeted toward the identification of species-specific markers for the clinically relevant M. tuberculosis and M. avium. In addition, HaeIII digestion of the amplification products yielded isolates-specific bands.
Original language | English |
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Pages (from-to) | 391-395 |
Number of pages | 5 |
Journal | New Microbiologica |
Volume | 21 |
Issue number | 4 |
Publication status | Published - Oct 1998 |
Keywords
- Mycobacteria identification
- Mycobacterium avium
- Mycobacterium tuberculosis
- rpoV gene
- Sigma factor
ASJC Scopus subject areas
- Microbiology
- Microbiology (medical)