Rapid identification of viable retrovirus-transduced cells using the green fluorescent protein as a marker

D. Klein, S. Indraccolo, K. Von Rombs, A. Amadori, B. Salmons, W. H. Günzburg

Research output: Contribution to journalArticle

Abstract

Various methods for determining the expression of the β-galactosidase (β-gal) gene after retroviral transduction were compared as a means to assess retroviral titre. To allow better comparison, different retroviral vectors were constructed carrying two mutants of the green fluorescent protein and assessed as sensitive markers of retroviral gene transfer. It could be demonstrated that GFP is generally superior to β-gal in terms of sensitivity, speed and non-invasiveness of assay, allowing easy direct FACS sorting of populations of transduced cells. This opens the possibility of enrichment by sorting of ex vivo transduced cells in gene therapy protocols.

Original languageEnglish
Pages (from-to)1256-1260
Number of pages5
JournalGene Therapy
Volume4
Issue number11
Publication statusPublished - 1997

Keywords

  • β-galactosidase
  • GFP
  • Retroviral vector
  • Titre

ASJC Scopus subject areas

  • Genetics

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