Rapid microscopy technique for detection of Pneumocystis carinii in fresh clinical specimens

G. Chichino, A. Bruno, C. Cevini, S. Gatti, M. Scaglia

Research output: Contribution to journalArticle

Abstract

A direct method for detection of Pneumocystis carinii was evaluated in 14 patients with impaired immune function (3 seropositive for HIV, 8 with AIDS and 3 with heart transplants) and signs and symptoms suggestive of Pneumocystis carinii pneumonia. Direct examination by phase-contrast and interference-contrast microscopy of fresh clinical specimens obtained by sputum induction, bronchoalveolar lavage or transbronchial lung biopsy was found to be a simple and rapid method for detection of Pneumocystis carinii, the sensitivity of the method being comparable to that of the classical toluidine blue O and Diff-Quik staining methods. These findings suggest that this direct microscopy technique could be considered for routine clinical application in patients with suspected Pneumocystis carinii pneumonia.

Original languageEnglish
Pages (from-to)601-605
Number of pages5
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 1990

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

Fingerprint Dive into the research topics of 'Rapid microscopy technique for detection of Pneumocystis carinii in fresh clinical specimens'. Together they form a unique fingerprint.

  • Cite this