Rapid modification of bacterial artificial chromosomes by ET-recombination

Joep P P Muyrers, Youming Zhang, Giuseppe Testa, A. Francis Stewart

Research output: Contribution to journalArticlepeer-review

Abstract

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

Original languageEnglish
Pages (from-to)1555-1557
Number of pages3
JournalNucleic Acids Research
Volume27
Issue number6
DOIs
Publication statusPublished - Mar 15 1999

ASJC Scopus subject areas

  • Genetics

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