Rapid potentiometric determination of cholinesterases in plasma and red cells

Application to eptastigmine monitoring

E. Cazzola, N. Lattuada, L. Zecca, D. Radice, M. Luzzana, B. P. Imbimbo, A. Auteri, A. Mosca

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Eptastigmine (MF 201) is a new physostigmine derivative with potent inhibitory activity on cholinesterases. Here we present a new potentiometric cholinesterase activity assay suitable for MF 201 monitoring. The analysis is performed on a differential pH system and has the following characteristics: (a) within-run precision: C.V. 2.0% (plasma cholinesterase), 1.8% (red cell cholinesterase); (b) between-run precision: C.V. 4.0% (plasma cholinesterase); (c) linearity: 1-10 kU/l (plasma cholinesterase), 6-70 U/g Hb (red cell cholinesterase); (d) comparison with a reference method (x, HITACHI 737 Boerhinger Mannheim, Italy): y = 0.785x - 0.07; n = 37; r = 0.998. The assay has been applied to the determination of plasma and red cell cholinesterase activity in volunteers over 60 years of age treated with a single oral dose of 30 mg eptastigmine. We found that red cell cholinesterase is selectively inhibited after MF 201 administration with the following kinetics (time, % of inhibition, mean ± S.E., n = 6): 0 h, 0; 1 h, 17 ± 4.6; 2 h, 24 ± 4; 4 h, 23 ± 4.4; 12 h, 14 ± 3. Eptastigmine plasma levels were also determined by a HPLC method: maximum concentration was found one hour after drug administration.

Original languageEnglish
Pages (from-to)265-268
Number of pages4
JournalChemico-Biological Interactions
Volume87
Issue number1-3
DOIs
Publication statusPublished - 1993

Fingerprint

Cholinesterases
Plasma Cells
Cells
Plasmas
Monitoring
Assays
physostigmine heptyl
Physostigmine
Italy
Volunteers
High Pressure Liquid Chromatography
Derivatives
Kinetics

Keywords

  • Acetylcholinesterase
  • Cholinesterase inhibitors
  • Differential pH
  • Erythrocytes

ASJC Scopus subject areas

  • Toxicology

Cite this

Rapid potentiometric determination of cholinesterases in plasma and red cells : Application to eptastigmine monitoring. / Cazzola, E.; Lattuada, N.; Zecca, L.; Radice, D.; Luzzana, M.; Imbimbo, B. P.; Auteri, A.; Mosca, A.

In: Chemico-Biological Interactions, Vol. 87, No. 1-3, 1993, p. 265-268.

Research output: Contribution to journalArticle

Cazzola, E. ; Lattuada, N. ; Zecca, L. ; Radice, D. ; Luzzana, M. ; Imbimbo, B. P. ; Auteri, A. ; Mosca, A. / Rapid potentiometric determination of cholinesterases in plasma and red cells : Application to eptastigmine monitoring. In: Chemico-Biological Interactions. 1993 ; Vol. 87, No. 1-3. pp. 265-268.
@article{fa1653182ffb46aabe753150273bda11,
title = "Rapid potentiometric determination of cholinesterases in plasma and red cells: Application to eptastigmine monitoring",
abstract = "Eptastigmine (MF 201) is a new physostigmine derivative with potent inhibitory activity on cholinesterases. Here we present a new potentiometric cholinesterase activity assay suitable for MF 201 monitoring. The analysis is performed on a differential pH system and has the following characteristics: (a) within-run precision: C.V. 2.0{\%} (plasma cholinesterase), 1.8{\%} (red cell cholinesterase); (b) between-run precision: C.V. 4.0{\%} (plasma cholinesterase); (c) linearity: 1-10 kU/l (plasma cholinesterase), 6-70 U/g Hb (red cell cholinesterase); (d) comparison with a reference method (x, HITACHI 737 Boerhinger Mannheim, Italy): y = 0.785x - 0.07; n = 37; r = 0.998. The assay has been applied to the determination of plasma and red cell cholinesterase activity in volunteers over 60 years of age treated with a single oral dose of 30 mg eptastigmine. We found that red cell cholinesterase is selectively inhibited after MF 201 administration with the following kinetics (time, {\%} of inhibition, mean ± S.E., n = 6): 0 h, 0; 1 h, 17 ± 4.6; 2 h, 24 ± 4; 4 h, 23 ± 4.4; 12 h, 14 ± 3. Eptastigmine plasma levels were also determined by a HPLC method: maximum concentration was found one hour after drug administration.",
keywords = "Acetylcholinesterase, Cholinesterase inhibitors, Differential pH, Erythrocytes",
author = "E. Cazzola and N. Lattuada and L. Zecca and D. Radice and M. Luzzana and Imbimbo, {B. P.} and A. Auteri and A. Mosca",
year = "1993",
doi = "10.1016/0009-2797(93)90053-2",
language = "English",
volume = "87",
pages = "265--268",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",
number = "1-3",

}

TY - JOUR

T1 - Rapid potentiometric determination of cholinesterases in plasma and red cells

T2 - Application to eptastigmine monitoring

AU - Cazzola, E.

AU - Lattuada, N.

AU - Zecca, L.

AU - Radice, D.

AU - Luzzana, M.

AU - Imbimbo, B. P.

AU - Auteri, A.

AU - Mosca, A.

PY - 1993

Y1 - 1993

N2 - Eptastigmine (MF 201) is a new physostigmine derivative with potent inhibitory activity on cholinesterases. Here we present a new potentiometric cholinesterase activity assay suitable for MF 201 monitoring. The analysis is performed on a differential pH system and has the following characteristics: (a) within-run precision: C.V. 2.0% (plasma cholinesterase), 1.8% (red cell cholinesterase); (b) between-run precision: C.V. 4.0% (plasma cholinesterase); (c) linearity: 1-10 kU/l (plasma cholinesterase), 6-70 U/g Hb (red cell cholinesterase); (d) comparison with a reference method (x, HITACHI 737 Boerhinger Mannheim, Italy): y = 0.785x - 0.07; n = 37; r = 0.998. The assay has been applied to the determination of plasma and red cell cholinesterase activity in volunteers over 60 years of age treated with a single oral dose of 30 mg eptastigmine. We found that red cell cholinesterase is selectively inhibited after MF 201 administration with the following kinetics (time, % of inhibition, mean ± S.E., n = 6): 0 h, 0; 1 h, 17 ± 4.6; 2 h, 24 ± 4; 4 h, 23 ± 4.4; 12 h, 14 ± 3. Eptastigmine plasma levels were also determined by a HPLC method: maximum concentration was found one hour after drug administration.

AB - Eptastigmine (MF 201) is a new physostigmine derivative with potent inhibitory activity on cholinesterases. Here we present a new potentiometric cholinesterase activity assay suitable for MF 201 monitoring. The analysis is performed on a differential pH system and has the following characteristics: (a) within-run precision: C.V. 2.0% (plasma cholinesterase), 1.8% (red cell cholinesterase); (b) between-run precision: C.V. 4.0% (plasma cholinesterase); (c) linearity: 1-10 kU/l (plasma cholinesterase), 6-70 U/g Hb (red cell cholinesterase); (d) comparison with a reference method (x, HITACHI 737 Boerhinger Mannheim, Italy): y = 0.785x - 0.07; n = 37; r = 0.998. The assay has been applied to the determination of plasma and red cell cholinesterase activity in volunteers over 60 years of age treated with a single oral dose of 30 mg eptastigmine. We found that red cell cholinesterase is selectively inhibited after MF 201 administration with the following kinetics (time, % of inhibition, mean ± S.E., n = 6): 0 h, 0; 1 h, 17 ± 4.6; 2 h, 24 ± 4; 4 h, 23 ± 4.4; 12 h, 14 ± 3. Eptastigmine plasma levels were also determined by a HPLC method: maximum concentration was found one hour after drug administration.

KW - Acetylcholinesterase

KW - Cholinesterase inhibitors

KW - Differential pH

KW - Erythrocytes

UR - http://www.scopus.com/inward/record.url?scp=0027177781&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027177781&partnerID=8YFLogxK

U2 - 10.1016/0009-2797(93)90053-2

DO - 10.1016/0009-2797(93)90053-2

M3 - Article

VL - 87

SP - 265

EP - 268

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

IS - 1-3

ER -