Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing

Fabio Arena, P. Alexander Rolfe, Graeme Doran, Viola Conte, Sarah Gruszka, Thomas Clarke, Yemi Adesokan, Tommaso Giani, Gian Maria Rossolini

Research output: Contribution to journalArticlepeer-review

Abstract

Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.

Original languageEnglish
Pages (from-to)987-990
Number of pages4
JournalJournal of Clinical Microbiology
Volume52
Issue number3
DOIs
Publication statusPublished - 2014

ASJC Scopus subject areas

  • Microbiology (medical)

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