Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing

Fabio Arena; P. Alexander Rolfe; Graeme Doran; Viola Conte; Sarah Gruszka; Thomas Clarke; Yemi Adesokan; Tommaso Giani; Gian Maria Rossolini

doi:10.1128/JCM.03247-13

Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing

Fabio Arena, P. Alexander Rolfe, Graeme Doran, Viola Conte, Sarah Gruszka, Thomas Clarke, Yemi Adesokan, Tommaso Giani, Gian Maria Rossolini

Fondazione Don Carlo Gnocchi Onlus

Research output: Contribution to journal › Article › peer-review

Abstract

Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.

Original language	English
Pages (from-to)	987-990
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1128/JCM.03247-13
Publication status	Published - 2014

ASJC Scopus subject areas

Microbiology (medical)

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Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing. / Arena, Fabio; Rolfe, P. Alexander; Doran, Graeme; Conte, Viola; Gruszka, Sarah; Clarke, Thomas; Adesokan, Yemi; Giani, Tommaso; Rossolini, Gian Maria.

In: Journal of Clinical Microbiology, Vol. 52, No. 3, 2014, p. 987-990.

Research output: Contribution to journal › Article › peer-review

Arena, Fabio ; Rolfe, P. Alexander ; Doran, Graeme ; Conte, Viola ; Gruszka, Sarah ; Clarke, Thomas ; Adesokan, Yemi ; Giani, Tommaso ; Rossolini, Gian Maria. / Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing. In: Journal of Clinical Microbiology. 2014 ; Vol. 52, No. 3. pp. 987-990.

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AU - Adesokan, Yemi

AU - Giani, Tommaso

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