TY - JOUR
T1 - Rapid resistome fingerprinting and clonal lineage profiling of carbapenem-resistant Klebsiella pneumoniae isolates by targeted next-generation sequencing
AU - Arena, Fabio
AU - Rolfe, P. Alexander
AU - Doran, Graeme
AU - Conte, Viola
AU - Gruszka, Sarah
AU - Clarke, Thomas
AU - Adesokan, Yemi
AU - Giani, Tommaso
AU - Rossolini, Gian Maria
PY - 2014
Y1 - 2014
N2 - Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.
AB - Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant β-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.
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U2 - 10.1128/JCM.03247-13
DO - 10.1128/JCM.03247-13
M3 - Article
C2 - 24403299
AN - SCOPUS:84894586819
VL - 52
SP - 987
EP - 990
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 3
ER -