Abstract
The substitution of codon 43 in the gene rpsL is the single most common mutation found in streptomycin-resistant Mycobacterium tuberculosis. The characterization of this mutation has been hampered by the need for prior cultivation of the mycobacteria, the need for DNA sequencing, or both. In this report we describe a simple and culture-independent technique to detect this mutation directly from sputum samples, requiring little more than a polymerase chain reaction (PCR) machine and a simple agarose minigel. There is no need for labeled probes or DNA sequencing. In a preliminary test of feasibility, interpretable results were obtained from all of 16 smear-positive and 1 of 4 smear-negative, culture-positive samples. Two of two samples containing M. tuberculosis with rpsL codon 43 mutations were correctly identified.
| Original language | English |
|---|---|
| Pages (from-to) | 56-60 |
| Number of pages | 5 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 63 |
| Issue number | 1-2 |
| Publication status | Published - 2000 |
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ASJC Scopus subject areas
- Parasitology
- Infectious Diseases
Cite this
Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis. / Mieskes, K. T.; Rüsch-Gerdes, S.; Truffot-Pernot, C.; Feldmann, K.; Tortoli, E.; Casal, M.; Löscher, T.; Rinder, H.
In: American Journal of Tropical Medicine and Hygiene, Vol. 63, No. 1-2, 2000, p. 56-60.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis
AU - Mieskes, K. T.
AU - Rüsch-Gerdes, S.
AU - Truffot-Pernot, C.
AU - Feldmann, K.
AU - Tortoli, E.
AU - Casal, M.
AU - Löscher, T.
AU - Rinder, H.
PY - 2000
Y1 - 2000
N2 - The substitution of codon 43 in the gene rpsL is the single most common mutation found in streptomycin-resistant Mycobacterium tuberculosis. The characterization of this mutation has been hampered by the need for prior cultivation of the mycobacteria, the need for DNA sequencing, or both. In this report we describe a simple and culture-independent technique to detect this mutation directly from sputum samples, requiring little more than a polymerase chain reaction (PCR) machine and a simple agarose minigel. There is no need for labeled probes or DNA sequencing. In a preliminary test of feasibility, interpretable results were obtained from all of 16 smear-positive and 1 of 4 smear-negative, culture-positive samples. Two of two samples containing M. tuberculosis with rpsL codon 43 mutations were correctly identified.
AB - The substitution of codon 43 in the gene rpsL is the single most common mutation found in streptomycin-resistant Mycobacterium tuberculosis. The characterization of this mutation has been hampered by the need for prior cultivation of the mycobacteria, the need for DNA sequencing, or both. In this report we describe a simple and culture-independent technique to detect this mutation directly from sputum samples, requiring little more than a polymerase chain reaction (PCR) machine and a simple agarose minigel. There is no need for labeled probes or DNA sequencing. In a preliminary test of feasibility, interpretable results were obtained from all of 16 smear-positive and 1 of 4 smear-negative, culture-positive samples. Two of two samples containing M. tuberculosis with rpsL codon 43 mutations were correctly identified.
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UR - http://www.scopus.com/inward/citedby.url?scp=0034445891&partnerID=8YFLogxK
M3 - Article
VL - 63
SP - 56
EP - 60
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 1-2
ER -