Rapid solid-phase extraction method for automated gas chromatographic- mass spectrometric determination of nicotine in plasma

Enrico Davoli, Luca Stramare, Roberto Fanelli, Luisa Diomede, Mario Salmona

Research output: Contribution to journalArticle

Abstract

The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.

Original languageEnglish
Pages (from-to)312-316
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume707
Issue number1-2
DOIs
Publication statusPublished - Apr 10 1998

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Nicotine
Gases
Plasmas
Plasma Gases
Tobacco Use Cessation Products
Deuterium
Assays
Chemical analysis

Keywords

  • Nicotine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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abstract = "The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3{\%} to 1.64{\%} (R.S.D.) depending on the concentration, while the deviation was 4.16{\%}. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.",
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AU - Davoli, Enrico

AU - Stramare, Luca

AU - Fanelli, Roberto

AU - Diomede, Luisa

AU - Salmona, Mario

PY - 1998/4/10

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AB - The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.

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