Abstract
The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
| Original language | English |
|---|---|
| Pages (from-to) | 312-316 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 707 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Apr 10 1998 |
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Keywords
- Nicotine
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Rapid solid-phase extraction method for automated gas chromatographic- mass spectrometric determination of nicotine in plasma. / Davoli, Enrico; Stramare, Luca; Fanelli, Roberto; Diomede, Luisa; Salmona, Mario.
In: Journal of Chromatography B: Biomedical Applications, Vol. 707, No. 1-2, 10.04.1998, p. 312-316.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rapid solid-phase extraction method for automated gas chromatographic- mass spectrometric determination of nicotine in plasma
AU - Davoli, Enrico
AU - Stramare, Luca
AU - Fanelli, Roberto
AU - Diomede, Luisa
AU - Salmona, Mario
PY - 1998/4/10
Y1 - 1998/4/10
N2 - The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
AB - The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
KW - Nicotine
UR - http://www.scopus.com/inward/record.url?scp=0032502953&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032502953&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00579-3
DO - 10.1016/S0378-4347(97)00579-3
M3 - Article
C2 - 9613965
AN - SCOPUS:0032502953
VL - 707
SP - 312
EP - 316
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1-2
ER -