TY - JOUR
T1 - Rapid solid-phase extraction method for automated gas chromatographic- mass spectrometric determination of nicotine in plasma
AU - Davoli, Enrico
AU - Stramare, Luca
AU - Fanelli, Roberto
AU - Diomede, Luisa
AU - Salmona, Mario
PY - 1998/4/10
Y1 - 1998/4/10
N2 - The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
AB - The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
KW - Nicotine
UR - http://www.scopus.com/inward/record.url?scp=0032502953&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032502953&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(97)00579-3
DO - 10.1016/S0378-4347(97)00579-3
M3 - Article
C2 - 9613965
AN - SCOPUS:0032502953
VL - 707
SP - 312
EP - 316
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 1387-2273
IS - 1-2
ER -