Abstract
The aim of this study was to develop a simple, rapid and sensitive assay of nicotine in plasma for automated gas chromatographic-mass spectrometric analysis. Biological samples were extracted using pre-packed Extrelut-1 columns with 5 ml of ethyl acetate. Quantitative analysis was done using deuterium-labelled nicotine as internal standard. The limit of quantitation was 0.5 ng in 1-ml plasma samples. Precision was ranging from 13.3% to 1.64% (R.S.D.) depending on the concentration, while the deviation was 4.16%. This method has been used for determination of nicotine bioavailability from new, low-dosage, nicotine chewing gum strips.
| Original language | English |
|---|---|
| Pages (from-to) | 312-316 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 707 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - Apr 10 1998 |
Keywords
- Nicotine
ASJC Scopus subject areas
- Chemistry(all)
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Cite this
Davoli, E., Stramare, L., Fanelli, R., Diomede, L., & Salmona, M. (1998). Rapid solid-phase extraction method for automated gas chromatographic- mass spectrometric determination of nicotine in plasma. Journal of Chromatography B: Biomedical Applications, 707(1-2), 312-316. https://doi.org/10.1016/S0378-4347(97)00579-3