Ras antagonizes cAMP stimulated glucagon gene transcription in pancreatic islet cell lines

Roberto Gherzi, Paola Briata, Hans Christoph Fehmann, Burkhard Göke

Research output: Contribution to journalArticle

Abstract

Ras, a GTP-binding protein, converts membrane tyrosine kinase signalling to changes in gene expression patterns. Utilising a rat glucagon promoter-CAT construct (p[-1.1]GLU-CAT) we demonstrate in transient transfection experiments that the oncogenic Ras inhibits cAMP-dependent activation of p[-1.1]GLU-CAT in both glucagonoma InR1-G9 and insulinoma β-TC1 cells. Conversely, the expression of a dominant negative mutant of Ras enhances the cAMP-induced activation of p[-1.1]GLU-CAT transcription in these cells. Our data suggests a functional interference of Ras with the cAMP-dependent transcription of the glucagon gene.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalFEBS Letters
Volume353
Issue number3
DOIs
Publication statusPublished - Oct 24 1994

Keywords

  • Gene transcription
  • Glucagon gene
  • Glucagonoma
  • Insulinoma
  • Protein kinase A
  • Ras

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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