Abstract
Ras, a GTP-binding protein, converts membrane tyrosine kinase signalling to changes in gene expression patterns. Utilising a rat glucagon promoter-CAT construct (p[-1.1]GLU-CAT) we demonstrate in transient transfection experiments that the oncogenic Ras inhibits cAMP-dependent activation of p[-1.1]GLU-CAT in both glucagonoma InR1-G9 and insulinoma β-TC1 cells. Conversely, the expression of a dominant negative mutant of Ras enhances the cAMP-induced activation of p[-1.1]GLU-CAT transcription in these cells. Our data suggests a functional interference of Ras with the cAMP-dependent transcription of the glucagon gene.
Original language | English |
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Pages (from-to) | 277-280 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 353 |
Issue number | 3 |
DOIs | |
Publication status | Published - Oct 24 1994 |
Keywords
- Gene transcription
- Glucagon gene
- Glucagonoma
- Insulinoma
- Protein kinase A
- Ras
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology