Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells

G. D. Borasio, A. Markus, R. Heumann, C. Ghezzi, A. Sampietro, A. Wittinghofer, V. Silani

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We have previously shown that the oncogene product p21Ras is essential for the survival and neurite outgrowth-promoting activity of nerve growth factor on cultured chick embryonic sensory, but not sympathetic neurons. In order to extend our observations to the human system and to non-neuronal cells, we introduced the oncogenic form of p21Ras into the cytoplasm of three different types of cultured human embryonic neural crest derivatives (8th-11th gestational week): dorsal root ganglion neurons, sympathetic neurons, and adrenal chromaffin cells. These cells are dependent on nerve growth factor for survival and/or fibre outgrowth in vitro. In dorsal root ganglion neurons, p21Ras promoted survival and fibre outgrowth which was quantitatively and qualitatively comparable to the nerve growth factor effect (84% vs. 95%, control 18%). Sympathetic neurons showed a similar effect, albeit with a higher background survival (91% vs. 93%, control 58%). On chromaffin cells, which respond to nerve growth factor with pronounced fibre outgrowth in culture, the effect of p21Ras was again comparable to that of nerve growth factor (35% vs. 30%, control 5%). The survival and fibre outgrowth-promoting effects of p21Ras on human embryonic dorsal root ganglion neurons, sympathetic neurons and chromaffin cells suggest an involvement of p21Ras in the intracellular signal transduction of nerve growth factor in human neural crest-derived cell populations.

Original languageEnglish
Pages (from-to)1121-1127
Number of pages7
JournalNeuroscience
Volume73
Issue number4
DOIs
Publication statusPublished - Aug 1996

Fingerprint

ras Proteins
Neural Crest
Nerve Growth Factor
Neurons
Survival
Chromaffin Cells
Spinal Ganglia
Oncogene Proteins
Signal Transduction
Cytoplasm
Population

Keywords

  • chromaffin cells
  • dorsal root ganglion neurons
  • nerve growth factor
  • p75 low affinity NGF receptor
  • signal transduction
  • sympathetic neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Borasio, G. D., Markus, A., Heumann, R., Ghezzi, C., Sampietro, A., Wittinghofer, A., & Silani, V. (1996). Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells. Neuroscience, 73(4), 1121-1127. https://doi.org/10.1016/0306-4522(96)00084-X

Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells. / Borasio, G. D.; Markus, A.; Heumann, R.; Ghezzi, C.; Sampietro, A.; Wittinghofer, A.; Silani, V.

In: Neuroscience, Vol. 73, No. 4, 08.1996, p. 1121-1127.

Research output: Contribution to journalArticle

Borasio, GD, Markus, A, Heumann, R, Ghezzi, C, Sampietro, A, Wittinghofer, A & Silani, V 1996, 'Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells', Neuroscience, vol. 73, no. 4, pp. 1121-1127. https://doi.org/10.1016/0306-4522(96)00084-X
Borasio GD, Markus A, Heumann R, Ghezzi C, Sampietro A, Wittinghofer A et al. Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells. Neuroscience. 1996 Aug;73(4):1121-1127. https://doi.org/10.1016/0306-4522(96)00084-X
Borasio, G. D. ; Markus, A. ; Heumann, R. ; Ghezzi, C. ; Sampietro, A. ; Wittinghofer, A. ; Silani, V. / Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells. In: Neuroscience. 1996 ; Vol. 73, No. 4. pp. 1121-1127.
@article{a1f942105b004144ab59d6f81cdc90bb,
title = "Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells",
abstract = "We have previously shown that the oncogene product p21Ras is essential for the survival and neurite outgrowth-promoting activity of nerve growth factor on cultured chick embryonic sensory, but not sympathetic neurons. In order to extend our observations to the human system and to non-neuronal cells, we introduced the oncogenic form of p21Ras into the cytoplasm of three different types of cultured human embryonic neural crest derivatives (8th-11th gestational week): dorsal root ganglion neurons, sympathetic neurons, and adrenal chromaffin cells. These cells are dependent on nerve growth factor for survival and/or fibre outgrowth in vitro. In dorsal root ganglion neurons, p21Ras promoted survival and fibre outgrowth which was quantitatively and qualitatively comparable to the nerve growth factor effect (84{\%} vs. 95{\%}, control 18{\%}). Sympathetic neurons showed a similar effect, albeit with a higher background survival (91{\%} vs. 93{\%}, control 58{\%}). On chromaffin cells, which respond to nerve growth factor with pronounced fibre outgrowth in culture, the effect of p21Ras was again comparable to that of nerve growth factor (35{\%} vs. 30{\%}, control 5{\%}). The survival and fibre outgrowth-promoting effects of p21Ras on human embryonic dorsal root ganglion neurons, sympathetic neurons and chromaffin cells suggest an involvement of p21Ras in the intracellular signal transduction of nerve growth factor in human neural crest-derived cell populations.",
keywords = "chromaffin cells, dorsal root ganglion neurons, nerve growth factor, p75 low affinity NGF receptor, signal transduction, sympathetic neurons",
author = "Borasio, {G. D.} and A. Markus and R. Heumann and C. Ghezzi and A. Sampietro and A. Wittinghofer and V. Silani",
year = "1996",
month = "8",
doi = "10.1016/0306-4522(96)00084-X",
language = "English",
volume = "73",
pages = "1121--1127",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Ras p21 protein promotes survival and differentiation of human embryonic neural crest-derived cells

AU - Borasio, G. D.

AU - Markus, A.

AU - Heumann, R.

AU - Ghezzi, C.

AU - Sampietro, A.

AU - Wittinghofer, A.

AU - Silani, V.

PY - 1996/8

Y1 - 1996/8

N2 - We have previously shown that the oncogene product p21Ras is essential for the survival and neurite outgrowth-promoting activity of nerve growth factor on cultured chick embryonic sensory, but not sympathetic neurons. In order to extend our observations to the human system and to non-neuronal cells, we introduced the oncogenic form of p21Ras into the cytoplasm of three different types of cultured human embryonic neural crest derivatives (8th-11th gestational week): dorsal root ganglion neurons, sympathetic neurons, and adrenal chromaffin cells. These cells are dependent on nerve growth factor for survival and/or fibre outgrowth in vitro. In dorsal root ganglion neurons, p21Ras promoted survival and fibre outgrowth which was quantitatively and qualitatively comparable to the nerve growth factor effect (84% vs. 95%, control 18%). Sympathetic neurons showed a similar effect, albeit with a higher background survival (91% vs. 93%, control 58%). On chromaffin cells, which respond to nerve growth factor with pronounced fibre outgrowth in culture, the effect of p21Ras was again comparable to that of nerve growth factor (35% vs. 30%, control 5%). The survival and fibre outgrowth-promoting effects of p21Ras on human embryonic dorsal root ganglion neurons, sympathetic neurons and chromaffin cells suggest an involvement of p21Ras in the intracellular signal transduction of nerve growth factor in human neural crest-derived cell populations.

AB - We have previously shown that the oncogene product p21Ras is essential for the survival and neurite outgrowth-promoting activity of nerve growth factor on cultured chick embryonic sensory, but not sympathetic neurons. In order to extend our observations to the human system and to non-neuronal cells, we introduced the oncogenic form of p21Ras into the cytoplasm of three different types of cultured human embryonic neural crest derivatives (8th-11th gestational week): dorsal root ganglion neurons, sympathetic neurons, and adrenal chromaffin cells. These cells are dependent on nerve growth factor for survival and/or fibre outgrowth in vitro. In dorsal root ganglion neurons, p21Ras promoted survival and fibre outgrowth which was quantitatively and qualitatively comparable to the nerve growth factor effect (84% vs. 95%, control 18%). Sympathetic neurons showed a similar effect, albeit with a higher background survival (91% vs. 93%, control 58%). On chromaffin cells, which respond to nerve growth factor with pronounced fibre outgrowth in culture, the effect of p21Ras was again comparable to that of nerve growth factor (35% vs. 30%, control 5%). The survival and fibre outgrowth-promoting effects of p21Ras on human embryonic dorsal root ganglion neurons, sympathetic neurons and chromaffin cells suggest an involvement of p21Ras in the intracellular signal transduction of nerve growth factor in human neural crest-derived cell populations.

KW - chromaffin cells

KW - dorsal root ganglion neurons

KW - nerve growth factor

KW - p75 low affinity NGF receptor

KW - signal transduction

KW - sympathetic neurons

UR - http://www.scopus.com/inward/record.url?scp=0029949292&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029949292&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(96)00084-X

DO - 10.1016/0306-4522(96)00084-X

M3 - Article

VL - 73

SP - 1121

EP - 1127

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 4

ER -