Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro: The effect of nerve growth factor

Hideyuki Kobayashi; Futoshi Izumi; Jacopo Meldolesi

doi:10.1016/0304-3940(86)90130-8

Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro: The effect of nerve growth factor

Hideyuki Kobayashi, Futoshi Izumi, Jacopo Meldolesi

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

Original language	English
Pages (from-to)	114-118
Number of pages	5
Journal	Neuroscience Letters
Volume	65
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(86)90130-8
Publication status	Published - Mar 28 1986

Keywords

adrenal chromaffin cell
nerve growth factor
noradrenaline release
α-latrotoxin

ASJC Scopus subject areas

Neuroscience(all)

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title = "Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro: The effect of nerve growth factor",

abstract = "Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.",

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AU - Izumi, Futoshi

AU - Meldolesi, Jacopo

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Y1 - 1986/3/28

N2 - Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

AB - Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

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