Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro: The effect of nerve growth factor

Hideyuki Kobayashi, Futoshi Izumi, Jacopo Meldolesi

Research output: Contribution to journalArticlepeer-review

Abstract

Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalNeuroscience Letters
Volume65
Issue number1
DOIs
Publication statusPublished - Mar 28 1986

Keywords

  • adrenal chromaffin cell
  • nerve growth factor
  • noradrenaline release
  • α-latrotoxin

ASJC Scopus subject areas

  • Neuroscience(all)

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