Abstract
Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.
| Original language | English |
|---|---|
| Pages (from-to) | 114-118 |
| Number of pages | 5 |
| Journal | Neuroscience Letters |
| Volume | 65 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 28 1986 |
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Keywords
- adrenal chromaffin cell
- nerve growth factor
- noradrenaline release
- α-latrotoxin
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro : The effect of nerve growth factor. / Kobayashi, Hideyuki; Izumi, Futoshi; Meldolesi, Jacopo.
In: Neuroscience Letters, Vol. 65, No. 1, 28.03.1986, p. 114-118.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro
T2 - The effect of nerve growth factor
AU - Kobayashi, Hideyuki
AU - Izumi, Futoshi
AU - Meldolesi, Jacopo
PY - 1986/3/28
Y1 - 1986/3/28
N2 - Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.
AB - Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.
KW - adrenal chromaffin cell
KW - nerve growth factor
KW - noradrenaline release
KW - α-latrotoxin
UR - http://www.scopus.com/inward/record.url?scp=0022619551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022619551&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(86)90130-8
DO - 10.1016/0304-3940(86)90130-8
M3 - Article
VL - 65
SP - 114
EP - 118
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -