Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro

The effect of nerve growth factor

Hideyuki Kobayashi, Futoshi Izumi, Jacopo Meldolesi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Previous studies showed that in the intact rat chromaffin cells are insensitive to the presynaptic stimulatory toxin, α-latrotoxin (α-LTx). In contrast, primary cultures of rat adrenal chromaffin cells respond to α-LTx by releasing stored catecholamines. The toxin-induced response was greater in cultures from young (8-10-day-old) than adult (40-50-day-old) animals, and increased when the time of culturing was prolonged from 4 to 20 days. Even greater responses were observed in cells cultured in the presence of nerve growth factor, a treatment which induces a neuronal-like phenotype. Acquisition of sensitivity to α-LTx appears to be an early marker of chromaffin cell commitment to differentiate towards a neuron.

Original languageEnglish
Pages (from-to)114-118
Number of pages5
JournalNeuroscience Letters
Volume65
Issue number1
DOIs
Publication statusPublished - Mar 28 1986

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Chromaffin Cells
Nerve Growth Factor
Catecholamines
Cultured Cells
Phenotype
Neurons
In Vitro Techniques

Keywords

  • adrenal chromaffin cell
  • nerve growth factor
  • noradrenaline release
  • α-latrotoxin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro : The effect of nerve growth factor. / Kobayashi, Hideyuki; Izumi, Futoshi; Meldolesi, Jacopo.

In: Neuroscience Letters, Vol. 65, No. 1, 28.03.1986, p. 114-118.

Research output: Contribution to journalArticle

Kobayashi, Hideyuki ; Izumi, Futoshi ; Meldolesi, Jacopo. / Rat adrenal chromaffin cells become sensitive to α-latrotoxin when cultured in vitro : The effect of nerve growth factor. In: Neuroscience Letters. 1986 ; Vol. 65, No. 1. pp. 114-118.
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