Rat blood leucocytes, unlike rabbit leucocytes, do not generate procoagulant activity on exposure to endotoxin

N. Semeraro, M. Colucci, L. Mussoni, M. B. Donati

Research output: Contribution to journalArticle

Abstract

We have investigated the ability of rat and rabbit leucocytes to generate procoagulant activity (PCA) in response to endotoxin in vitro and in vivo. On prolonged incubation with endotoxin (10 μg/ml f.c.) isolated rabbit leucocytes developed strong PCA as measured by clotting and amidolytic assay. In contrast, rat leucocytes failed to produce any PCA even in the presence of huge amounts of endotoxin (200 μg/ml f.c.). When rabbits were given two spaced endotoxin injections (25 μg/kg body wt 24 hr apart), blood leucocytes harvested 30-60 min after the second injection consistently showed marked PCA. Again, unlike the rabbit, rat leucocytes obtained after 2 endotoxin injections (up to 2 mg/kg body wt) showed absolutely no PCA. These findings support the view that leucocytes are involved in endotoxin-induced disseminated intravascular coagulation (DIC) in rabbits. On the other hand the poor response of rat leucocytes to endotoxin might help explain the resistance of rats to DIC and Sanarelli-Schwartzman reaction.

Original languageEnglish
Pages (from-to)638-642
Number of pages5
JournalBritish Journal of Experimental Pathology
Volume62
Issue number6
Publication statusPublished - 1981

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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