Abstract
A study has been made of the association properties of the two GM1 ganglioside molecular species GM1-C18 and GM1-C20 (containing C18 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to give a form of association, the trypsin-labile form. This form was removed by treatment with trypsin enzyme. Both gangliosides associated stably with the cells to become components of the cell membranes. Although similar amounts of the two gangliosides entered the cells, being then metabolized, the time course of the association was different for the two gangliosides: after 15 h of ganglioside-cell incubation the amount of GM1-C18 inserted into the cell membrane was 2.43 times higher than that of GM1-C20.
| Original language | English |
|---|---|
| Pages (from-to) | 247-252 |
| Number of pages | 6 |
| Journal | Chemistry and Physics of Lipids |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1992 |
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Keywords
- cell association
- cerebellar granule cells
- gangliosides
- long chain bases
- metabolism
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
Rat cerebellar granule cells in culture associate and metabolize differently exogenous GM1 ganglioside molecular species containing a C18 or C20 long chain base. / Valsecchi, Manuela; Chigorno, Vanna; Sonnino, Sandro; Tettamanti, Guido.
In: Chemistry and Physics of Lipids, Vol. 60, No. 3, 1992, p. 247-252.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rat cerebellar granule cells in culture associate and metabolize differently exogenous GM1 ganglioside molecular species containing a C18 or C20 long chain base
AU - Valsecchi, Manuela
AU - Chigorno, Vanna
AU - Sonnino, Sandro
AU - Tettamanti, Guido
PY - 1992
Y1 - 1992
N2 - A study has been made of the association properties of the two GM1 ganglioside molecular species GM1-C18 and GM1-C20 (containing C18 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to give a form of association, the trypsin-labile form. This form was removed by treatment with trypsin enzyme. Both gangliosides associated stably with the cells to become components of the cell membranes. Although similar amounts of the two gangliosides entered the cells, being then metabolized, the time course of the association was different for the two gangliosides: after 15 h of ganglioside-cell incubation the amount of GM1-C18 inserted into the cell membrane was 2.43 times higher than that of GM1-C20.
AB - A study has been made of the association properties of the two GM1 ganglioside molecular species GM1-C18 and GM1-C20 (containing C18 and C20 long chain bases, respectively) to rat cerebellar granule cells in culture. Both gangliosides recognized, to the same extent, and associated with them to give a form of association, the trypsin-labile form. This form was removed by treatment with trypsin enzyme. Both gangliosides associated stably with the cells to become components of the cell membranes. Although similar amounts of the two gangliosides entered the cells, being then metabolized, the time course of the association was different for the two gangliosides: after 15 h of ganglioside-cell incubation the amount of GM1-C18 inserted into the cell membrane was 2.43 times higher than that of GM1-C20.
KW - cell association
KW - cerebellar granule cells
KW - gangliosides
KW - long chain bases
KW - metabolism
UR - http://www.scopus.com/inward/record.url?scp=0026600013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026600013&partnerID=8YFLogxK
U2 - 10.1016/0009-3084(92)90076-2
DO - 10.1016/0009-3084(92)90076-2
M3 - Article
VL - 60
SP - 247
EP - 252
JO - Chemistry and Physics of Lipids
JF - Chemistry and Physics of Lipids
SN - 0009-3084
IS - 3
ER -