Rat coagulating gland secretion contains a kinesin heavy chain-like protein acting as a type iv transglutaminase substrate

C. Esposito, L. Mariniello, A. Cozzolino, A. Amoresano, S. Orrù, R. Porta

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Abstract

By a proteomic approach, we demonstrated in rat coagulating gland secretion the presence of a 120 kDa protein which shares at least 80% identity at the amino acid level with the most closely related kinesin heavy chain codified by the kinesin superfamily protein Kif5c gene. In addition, we identified 30 and 66 kDa proteolytic fragments of such a kinesin heavy chain-like protein, corresponding to the 73-299 N-terminal and 300-860 C-terminal regions, respectively. Finally, we demonstrated the occurrence in coagulating gland secretion of a 200 kDa protein probably derived by cross-linking reaction of the kinesin heavy chain-like protein with type IV transglutaminase. In fact, kinesin heavy chain-like protein and its 66 kDa proteolytic fragment were also found to act as effective acyl donor substrates for the enzyme in vitro.

Original languageEnglish
Pages (from-to)4966-4971
Number of pages6
JournalBiochemistry
Volume40
Issue number16
DOIs
Publication statusPublished - Apr 24 2001

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Esposito, C., Mariniello, L., Cozzolino, A., Amoresano, A., Orrù, S., & Porta, R. (2001). Rat coagulating gland secretion contains a kinesin heavy chain-like protein acting as a type iv transglutaminase substrate. Biochemistry, 40(16), 4966-4971. https://doi.org/10.1021/bi001542w