Rat model of perchloroethylene-induced renal dysfunctions

Enrico Bergamaschi, Antonio Mutti, Maria Concetta Bocchi, Rossella Alinovi, Giorgio Olivetti, Gian Marco Ghiggeri, Innocente Franchini

Research output: Contribution to journalArticle

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Abstract

To investigate whether perchloroethylene (PCE) can induce renal disturbances and to compare morphological alterations with functional data, two groups of 12 male and female Fischer-344 mature rats were treated daily with PCE (500 mg/kg body wt in corn oil, p.o.) for 4 weeks. Sex- and age-matched control groups received corn oil only. Weekly, the urinary excretion of albumin (Alb), α-globulin (α) and retinol-binding protein (RBP) was measured in 24-hr urine samples using immunoassays specific for rat proteins. N-acetylglucosaminidase (NAG) activity was measured by a colorimetric assay. Electrophoretic analysis of proteinuria included SDS-PAGE and isoelectric-focusing of Alb purified from serum and urine. Weekly histopathology comprised light and electron microscopy. In the male rat, a trend toward progressive albuminuria (up to 15 times the pair-fed controls) was oberved, together with transient increases in α and NAG; RBP showed a twofold increase at the end of treatment. Histopathology failed to demonstrate glomerular changes, whereas it displayed α accumulation and mild lesions in the S2 segment of proximal tubules. Thus, in the male rat, the selective damage to S2 was associated with "glomerular" proteinuria, the α cortical content being closely correlated with albuminuria (n = 9, r = 0.92, P <0.001). In the female rat, only minor, although statistically significant (P <0.05), increases were recorded for Alb, whereas urinary α reached up to four times the control values. As a whole, these findings suggest that PCE, like other hydrocarbons, selectively affects the tubular segment S2 in the rat. A competition with α for tubular uptake could explain enhanced albuminuria. Owing to the species specificity of α, caution should be exercised in extrapolating these findings to man.

Original languageEnglish
Pages (from-to)427-439
Number of pages13
JournalEnvironmental Research
Volume59
Issue number2
DOIs
Publication statusPublished - 1992

Fingerprint

Tetrachloroethylene
tetrachloroethylene
Rats
histopathology
Kidney
urine
Albuminuria
protein
maize
Albumins
Acetylglucosaminidase
Corn Oil
oil
immunoassay
electron microscopy
Proteinuria
lesion
excretion
microscopy
serum

ASJC Scopus subject areas

  • Environmental Science(all)

Cite this

Bergamaschi, E., Mutti, A., Bocchi, M. C., Alinovi, R., Olivetti, G., Ghiggeri, G. M., & Franchini, I. (1992). Rat model of perchloroethylene-induced renal dysfunctions. Environmental Research, 59(2), 427-439. https://doi.org/10.1016/S0013-9351(05)80046-5

Rat model of perchloroethylene-induced renal dysfunctions. / Bergamaschi, Enrico; Mutti, Antonio; Bocchi, Maria Concetta; Alinovi, Rossella; Olivetti, Giorgio; Ghiggeri, Gian Marco; Franchini, Innocente.

In: Environmental Research, Vol. 59, No. 2, 1992, p. 427-439.

Research output: Contribution to journalArticle

Bergamaschi, E, Mutti, A, Bocchi, MC, Alinovi, R, Olivetti, G, Ghiggeri, GM & Franchini, I 1992, 'Rat model of perchloroethylene-induced renal dysfunctions', Environmental Research, vol. 59, no. 2, pp. 427-439. https://doi.org/10.1016/S0013-9351(05)80046-5
Bergamaschi, Enrico ; Mutti, Antonio ; Bocchi, Maria Concetta ; Alinovi, Rossella ; Olivetti, Giorgio ; Ghiggeri, Gian Marco ; Franchini, Innocente. / Rat model of perchloroethylene-induced renal dysfunctions. In: Environmental Research. 1992 ; Vol. 59, No. 2. pp. 427-439.
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