Rat sensorimotor cortex tolerance to parallel transections induced by synchrotron-generated X-ray microbeams

Erminia Fardone, Alberto Bravin, Alfredo Conti, Elke Bräuer-Krisch, Herwig Requardt, Domenico Bucci, Geraldine Le Duc, Giuseppe Battaglia, Pantaleo Romanelli

Research output: Contribution to journalArticle

Abstract

Microbeam radiation therapy is a novel preclinical technique, which uses synchrotron-generated X-rays for the treatment of brain tumours and drug-resistant epilepsies. In order to safely translate this approach to humans, a more in-depth knowledge of the long-term radiobiology of microbeams in healthy tissues is required. We report here the result of the characterization of the rat sensorimotor cortex tolerance to microradiosurgical parallel transections. Healthy adult male Wistar rats underwent irradiation with arrays of parallel microbeams. Beam thickness, spacing and incident dose were 100 or 600 μm, 400 or 1200 μm and 360 or 150 Gy, respectively. Motor performance was carried over a 3-month period. Three months after irradiation rats were sacrificed to evaluate the effects of irradiation on brain tissues by histology and immunohistochemistry. Microbeam irradiation of sensorimotor cortex did not affect weight gain and motor performance. No gross signs of paralysis or paresis were also observed. The cortical architecture was not altered, despite the presence of cell death along the irradiation path. Reactive gliosis was evident in the microbeam path of rats irradiated with 150 Gy, whereas no increase was observed in rats irradiated with 360 Gy.

Original languageEnglish
Article number14290
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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Synchrotrons
X-Rays
Radiobiology
Gliosis
Paresis
Brain Neoplasms
Paralysis
Weight Gain
Wistar Rats
Histology
Cell Death
Radiotherapy
Immunohistochemistry
Sensorimotor Cortex
Brain
Therapeutics

ASJC Scopus subject areas

  • General

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Rat sensorimotor cortex tolerance to parallel transections induced by synchrotron-generated X-ray microbeams. / Fardone, Erminia; Bravin, Alberto; Conti, Alfredo; Bräuer-Krisch, Elke; Requardt, Herwig; Bucci, Domenico; Le Duc, Geraldine; Battaglia, Giuseppe; Romanelli, Pantaleo.

In: Scientific Reports, Vol. 7, No. 1, 14290, 01.12.2017.

Research output: Contribution to journalArticle

Fardone, Erminia ; Bravin, Alberto ; Conti, Alfredo ; Bräuer-Krisch, Elke ; Requardt, Herwig ; Bucci, Domenico ; Le Duc, Geraldine ; Battaglia, Giuseppe ; Romanelli, Pantaleo. / Rat sensorimotor cortex tolerance to parallel transections induced by synchrotron-generated X-ray microbeams. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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