Rat tenascin-R gene: Structure, chromosome location and transcriptional activity of promoter and exon 1

A. Leprini, R. Gherzi, E. Vecchi, L. Borsi, L. Zardi, A. Siri

Research output: Contribution to journalArticle

Abstract

Tenascin-R is an extracellular matrix protein expressed exclusively in the central nervous system where it is thought to play a relevant role in regulating neurite outgrowth. We have i) cloned the cDNA of the rat tenascin-R 5' region; ii) defined its genomic organization, obtaining the sequence of two novel untranslated exons; iii) mapped the gene to rat chromosome 13q23 and suggested a previously unreported synteny between rat chromosome 13q23, human chromosome 1q24, and mouse chromosome 4E; and iv) sequenced and characterized the elements responsible for its neural cell-restricted transcription. We found that two discrete regions of the rat gene (the first in the proximal promoter, the second in the first exon) are independently able to activate to a high degree the transcription of a reporter gene in either human or rat neuroblastoma cell lines but not in other cell lines. Based on this observation, we re-evaluated the arrangement of transcriptionally active regions in the human tenascin-R gene we recently cloned and found that the human gene also contains an exon sequence able to initiate and sustain transcription independently of promoter sequences.

Original languageEnglish
Pages (from-to)115-123
Number of pages9
JournalCytogenetics and Cell Genetics
Volume83
Issue number1-2
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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