Ratiometric-pericam-mt, a novel tool to evaluate intramitochondrial zinc

Teresa Caporale, Domenico Ciavardelli, Carmine Di Ilio, Paola Lanuti, Denise Drago, Stefano L. Sensi

Research output: Contribution to journalArticlepeer-review


Zn2+ can enter mitochondria and promote a plethora of physiological and patho-physiological effects. The issue of measuring changes in intramitochondrial levels is therefore critical. Past studies have employed fluorescent Zn2+ indicators, like Rhod-2 and RhodZin-3, however, the use of these probes is impaired by their extramitochondrial sequestration. In this study, we show that the ratiometric mitochondria-targeted pericam, RPmt, can be employed to detect changes of intramitochondrial free Zn2+ ([Zn2+]m) levels. Using RPmt in neuronal and non neuronal cell lines we demonstrate that mitochondria can take up the cation mobilized from the cytosolic pool of protein-bound Zn2+ and that mitochondrial Zn2+ sequestration is largely mediated by the activity of the Ca2+ uniporter.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalExperimental Neurology
Issue number2
Publication statusPublished - Aug 2009

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Neurology


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