TY - JOUR
T1 - Ratiometric-pericam-mt, a novel tool to evaluate intramitochondrial zinc
AU - Caporale, Teresa
AU - Ciavardelli, Domenico
AU - Di Ilio, Carmine
AU - Lanuti, Paola
AU - Drago, Denise
AU - Sensi, Stefano L.
PY - 2009/8
Y1 - 2009/8
N2 - Zn2+ can enter mitochondria and promote a plethora of physiological and patho-physiological effects. The issue of measuring changes in intramitochondrial levels is therefore critical. Past studies have employed fluorescent Zn2+ indicators, like Rhod-2 and RhodZin-3, however, the use of these probes is impaired by their extramitochondrial sequestration. In this study, we show that the ratiometric mitochondria-targeted pericam, RPmt, can be employed to detect changes of intramitochondrial free Zn2+ ([Zn2+]m) levels. Using RPmt in neuronal and non neuronal cell lines we demonstrate that mitochondria can take up the cation mobilized from the cytosolic pool of protein-bound Zn2+ and that mitochondrial Zn2+ sequestration is largely mediated by the activity of the Ca2+ uniporter.
AB - Zn2+ can enter mitochondria and promote a plethora of physiological and patho-physiological effects. The issue of measuring changes in intramitochondrial levels is therefore critical. Past studies have employed fluorescent Zn2+ indicators, like Rhod-2 and RhodZin-3, however, the use of these probes is impaired by their extramitochondrial sequestration. In this study, we show that the ratiometric mitochondria-targeted pericam, RPmt, can be employed to detect changes of intramitochondrial free Zn2+ ([Zn2+]m) levels. Using RPmt in neuronal and non neuronal cell lines we demonstrate that mitochondria can take up the cation mobilized from the cytosolic pool of protein-bound Zn2+ and that mitochondrial Zn2+ sequestration is largely mediated by the activity of the Ca2+ uniporter.
UR - http://www.scopus.com/inward/record.url?scp=67649794848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67649794848&partnerID=8YFLogxK
U2 - 10.1016/j.expneurol.2009.04.003
DO - 10.1016/j.expneurol.2009.04.003
M3 - Article
C2 - 19374897
AN - SCOPUS:67649794848
VL - 218
SP - 228
EP - 234
JO - Experimental Neurology
JF - Experimental Neurology
SN - 0014-4886
IS - 2
ER -