rC5a directs the in vitro migration of human memory and naive tonsillar B lymphocytes: Implications for B cell trafficking in secondary lymphoid tissues

Luciano Ottonello, Anna Corcione, Giuseppe Tortolina, Irma Airoldi, Emilia Albesiano, Anna Favre, Roberto D'Agostino, Fabio Malavasi, Vito Pistoia, Franco Dallegri

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Abstract

Human C5a is a potent chemoattractant for granulocytes, monocytes, and dendritic cells. In mice C5a has been shown to be chemotactic for germinal center (GC) B cells. To date, no information is available on the effects of C5a on human B cell locomotion. Here we demonstrate that rC5a increases polarization and migration of human tonsillar B cells. The locomotory response was due to both chemokinetic and chemotactic activities of rC5a. Moreover, memory and, at a lesser extent, naive B cell fractions from purified tonsillar populations displayed rC5a-enhanced migratory properties, whereas GC cells did not. Flow cytometry revealed C5aR (CD88) on approximately 40% memory and 10% naive cells, respectively, whereas GC cells were negative. Immunohistochemistry showed that a few CD88+ cells were of the B cell lineage and localized in tonsillar subepithelial areas, where the majority of memory B cells settle. Pretreatment of memory B cells with the CD88 mAb abolished their migratory responsiveness to rC5a. Finally, the C5 gene was found to be expressed in naive, GC, and memory B lymphocytes at both the mRNA and the protein level. This study delineates a novel role for C5a as a regulator of the trafficking of human memory and naive B lymphocytes and supports the hypothesis that the B cells themselves may serve as source of C5 in secondary lymphoid tissues.

Original languageEnglish
Pages (from-to)6510-6517
Number of pages8
JournalJournal of Immunology
Volume162
Issue number11
Publication statusPublished - Jun 1 1999

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Lymphoid Tissue
B-Lymphocytes
Germinal Center
Human Trafficking
In Vitro Techniques
Chemotactic Factors
Cell Lineage
Granulocytes
Dendritic Cells
Cell Movement
Monocytes
Flow Cytometry
Immunohistochemistry
Messenger RNA

ASJC Scopus subject areas

  • Immunology

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rC5a directs the in vitro migration of human memory and naive tonsillar B lymphocytes : Implications for B cell trafficking in secondary lymphoid tissues. / Ottonello, Luciano; Corcione, Anna; Tortolina, Giuseppe; Airoldi, Irma; Albesiano, Emilia; Favre, Anna; D'Agostino, Roberto; Malavasi, Fabio; Pistoia, Vito; Dallegri, Franco.

In: Journal of Immunology, Vol. 162, No. 11, 01.06.1999, p. 6510-6517.

Research output: Contribution to journalArticle

Ottonello, Luciano ; Corcione, Anna ; Tortolina, Giuseppe ; Airoldi, Irma ; Albesiano, Emilia ; Favre, Anna ; D'Agostino, Roberto ; Malavasi, Fabio ; Pistoia, Vito ; Dallegri, Franco. / rC5a directs the in vitro migration of human memory and naive tonsillar B lymphocytes : Implications for B cell trafficking in secondary lymphoid tissues. In: Journal of Immunology. 1999 ; Vol. 162, No. 11. pp. 6510-6517.
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