Reaction of human serum albumin with aldoses

Gian Marco Ghiggeri, Giovanni Candiano, Gerolamo Delfino, Carlo Queirolo, Giuseppe Vecchio, Elisabetta Gianazza, Pier Giorgio Righetti

Research output: Contribution to journalArticle

Abstract

The reaction of human serum albumin (HSA) with aldoses (C3-C6) and acetaldehyde has been studied. U.v. and fluorescent spectra of the HSA-glyceraldehyde and HSA-GlcN adducts reveal yellow chromophores absorbing at 300-350 nm and emitting at 435 nm. However, even limited reaction of HSA with acetaldehyde induced perturbation in the Trp microenvironment. C.d. spectra of the adducts show an average 20% decrement in mean residual ellipticity [θ], which is independent of the extent of the reaction and the aldose used. It is concluded that most of the reactions with aldoses occur at the surface of the HSA molecule. With the exception of the GlcN adduct, the HSA adducts rearrange to produce pyrrole rings on the protein surface. I.e.f. analysis shows that the pI values of the modified HSA are almost linearly correlated with the chain length of the reacting aldose: from pI 4.2 for HSA-glyceraldehyde up to pI 5.0 for HSA-GlcN.

Original languageEnglish
Pages (from-to)113-122
Number of pages10
JournalCarbohydrate Research
Volume145
Issue number1
DOIs
Publication statusPublished - Dec 15 1985

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry

Cite this

Ghiggeri, G. M., Candiano, G., Delfino, G., Queirolo, C., Vecchio, G., Gianazza, E., & Righetti, P. G. (1985). Reaction of human serum albumin with aldoses. Carbohydrate Research, 145(1), 113-122. https://doi.org/10.1016/S0008-6215(00)90417-8