Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells

Simona Damiano, Roberta Fusco, Annalisa Morano, Mariarosaria de Mizio, Roberto Paternò, Antonella de Rosa, Rosa Spinelli, Stefano Amente, Rodolfo Frunzio, Paolo Mondola, Francoise Miot, Paolo Laccetti, Mariarosaria Santillo, Enrico Vittorio Avvedimento

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Dual Oxidases (DUOX) 1 and 2 are efficiently expressed in thyroid, gut, lung and immune system. The function and the regulation of these enzymes in mammals are still largely unknown. We report here that DUOX 1 and 2 are expressed in human neuroblastoma SK-N-BE cells as well as in a human oligodendrocyte cell line (MO3-13) and in rat brain and they are induced by platelet derived growth factor (PDGF). The levels of DUOX 1 and 2 proteins and mRNAs are induced by reactive oxygen species (ROS) produced by the membrane NADPH oxidase. As to the mechanism, we find that PDGF stimulates membrane NADPH oxidase to produce ROS, which stabilize DUOX1 and 2 mRNAs and increases the levels of the proteins. Silencing of gp91 phox (NOX2), or of the other membrane subunit of NADPH oxidase, p22 phox, blocks PDGF induction of DUOX1 and 2. These data unravel a novel mechanism of regulation of DUOX enzymes by ROS and identify a circuitry linking NADPH oxidase activity to DUOX1 and 2 levels in neuroblastoma cells.

Original languageEnglish
Article numbere34405
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 16 2012

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NADPH Oxidase
Neuroblastoma
reactive oxygen species
Reactive Oxygen Species
Platelet-Derived Growth Factor
Oxidoreductases
Membranes
platelet-derived growth factor
cells
Messenger RNA
Mammals
Immune system
Oligodendroglia
Enzymes
Rats
Immune System
Brain
Thyroid Gland
Proteins
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Damiano, S., Fusco, R., Morano, A., de Mizio, M., Paternò, R., de Rosa, A., ... Avvedimento, E. V. (2012). Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells. PLoS One, 7(4), [e34405]. https://doi.org/10.1371/journal.pone.0034405

Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells. / Damiano, Simona; Fusco, Roberta; Morano, Annalisa; de Mizio, Mariarosaria; Paternò, Roberto; de Rosa, Antonella; Spinelli, Rosa; Amente, Stefano; Frunzio, Rodolfo; Mondola, Paolo; Miot, Francoise; Laccetti, Paolo; Santillo, Mariarosaria; Avvedimento, Enrico Vittorio.

In: PLoS One, Vol. 7, No. 4, e34405, 16.04.2012.

Research output: Contribution to journalArticle

Damiano, S, Fusco, R, Morano, A, de Mizio, M, Paternò, R, de Rosa, A, Spinelli, R, Amente, S, Frunzio, R, Mondola, P, Miot, F, Laccetti, P, Santillo, M & Avvedimento, EV 2012, 'Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells', PLoS One, vol. 7, no. 4, e34405. https://doi.org/10.1371/journal.pone.0034405
Damiano, Simona ; Fusco, Roberta ; Morano, Annalisa ; de Mizio, Mariarosaria ; Paternò, Roberto ; de Rosa, Antonella ; Spinelli, Rosa ; Amente, Stefano ; Frunzio, Rodolfo ; Mondola, Paolo ; Miot, Francoise ; Laccetti, Paolo ; Santillo, Mariarosaria ; Avvedimento, Enrico Vittorio. / Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells. In: PLoS One. 2012 ; Vol. 7, No. 4.
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