Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells

Simona Damiano; Roberta Fusco; Annalisa Morano; Mariarosaria de Mizio; Roberto Paternò; Antonella de Rosa; Rosa Spinelli; Stefano Amente; Rodolfo Frunzio; Paolo Mondola; Francoise Miot; Paolo Laccetti; Mariarosaria Santillo; Enrico Vittorio Avvedimento

doi:10.1371/journal.pone.0034405

Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells

Simona Damiano, Roberta Fusco, Annalisa Morano, Mariarosaria de Mizio, Roberto Paternò, Antonella de Rosa, Rosa Spinelli, Stefano Amente, Rodolfo Frunzio, Paolo Mondola, Francoise Miot, Paolo Laccetti, Mariarosaria Santillo, Enrico Vittorio Avvedimento

Istituto Nazionale Tumori Fondazione G. Pascale

Research output: Contribution to journal › Article › peer-review

Abstract

Dual Oxidases (DUOX) 1 and 2 are efficiently expressed in thyroid, gut, lung and immune system. The function and the regulation of these enzymes in mammals are still largely unknown. We report here that DUOX 1 and 2 are expressed in human neuroblastoma SK-N-BE cells as well as in a human oligodendrocyte cell line (MO3-13) and in rat brain and they are induced by platelet derived growth factor (PDGF). The levels of DUOX 1 and 2 proteins and mRNAs are induced by reactive oxygen species (ROS) produced by the membrane NADPH oxidase. As to the mechanism, we find that PDGF stimulates membrane NADPH oxidase to produce ROS, which stabilize DUOX1 and 2 mRNAs and increases the levels of the proteins. Silencing of gp91 ^phox (NOX2), or of the other membrane subunit of NADPH oxidase, p22 ^phox, blocks PDGF induction of DUOX1 and 2. These data unravel a novel mechanism of regulation of DUOX enzymes by ROS and identify a circuitry linking NADPH oxidase activity to DUOX1 and 2 levels in neuroblastoma cells.

Original language	English
Article number	e34405
Journal	PLoS One
Volume	7
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0034405
Publication status	Published - Apr 16 2012

ASJC Scopus subject areas

Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)

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Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells. / Damiano, Simona; Fusco, Roberta; Morano, Annalisa; de Mizio, Mariarosaria; Paternò, Roberto; de Rosa, Antonella; Spinelli, Rosa; Amente, Stefano; Frunzio, Rodolfo; Mondola, Paolo; Miot, Francoise; Laccetti, Paolo; Santillo, Mariarosaria; Avvedimento, Enrico Vittorio.

In: PLoS One, Vol. 7, No. 4, e34405, 16.04.2012.

Research output: Contribution to journal › Article › peer-review

Damiano, Simona ; Fusco, Roberta ; Morano, Annalisa ; de Mizio, Mariarosaria ; Paternò, Roberto ; de Rosa, Antonella ; Spinelli, Rosa ; Amente, Stefano ; Frunzio, Rodolfo ; Mondola, Paolo ; Miot, Francoise ; Laccetti, Paolo ; Santillo, Mariarosaria ; Avvedimento, Enrico Vittorio. / Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells. In: PLoS One. 2012 ; Vol. 7, No. 4.

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title = "Reactive oxygen species regulate the levels of dual oxidase (duox1-2) in human neuroblastoma cells",

abstract = "Dual Oxidases (DUOX) 1 and 2 are efficiently expressed in thyroid, gut, lung and immune system. The function and the regulation of these enzymes in mammals are still largely unknown. We report here that DUOX 1 and 2 are expressed in human neuroblastoma SK-N-BE cells as well as in a human oligodendrocyte cell line (MO3-13) and in rat brain and they are induced by platelet derived growth factor (PDGF). The levels of DUOX 1 and 2 proteins and mRNAs are induced by reactive oxygen species (ROS) produced by the membrane NADPH oxidase. As to the mechanism, we find that PDGF stimulates membrane NADPH oxidase to produce ROS, which stabilize DUOX1 and 2 mRNAs and increases the levels of the proteins. Silencing of gp91 phox (NOX2), or of the other membrane subunit of NADPH oxidase, p22 phox, blocks PDGF induction of DUOX1 and 2. These data unravel a novel mechanism of regulation of DUOX enzymes by ROS and identify a circuitry linking NADPH oxidase activity to DUOX1 and 2 levels in neuroblastoma cells.",

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AU - Mondola, Paolo

AU - Miot, Francoise

AU - Laccetti, Paolo

AU - Santillo, Mariarosaria

AU - Avvedimento, Enrico Vittorio

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